摘要
研究了水解淀粉乳酸细菌对厨余垃圾发酵生产乳酸的强化作用．从厌氧发酵的厨余垃圾中分离到6株水解淀粉的乳酸细菌，其中菌株FHl64具有最高的淀粉降解率和乳酸产量．在pH5．5～6．0条件下，经48h发酵，菌株FHl64从40．50g·L^-1的可溶性淀粉产生32．67g·L^-1的乳酸．根据形态、生理生化特征和16S rDNA序列分析结果，可将菌株FH164鉴定为链球菌属（Streptococcussp．）细菌．接种菌株FH164可强化厨余垃圾的乳酸发酵，采用垃圾不灭菌的开放式发酵，菌株FH164可得到28，23g·L^-1的乳酸，比自然发酵（不接种的对照）的乳酸浓度高19．2％．
in order to realize resource recycling of kitchen waste, the enhancement of fermentative production of lactic acid （LA） by a strain of amylolytic lactic acid bacterium （ALAB） isolated from kitchen waste was investigated. A total of 6 strains of ALAB were isolated from anaerobically fermented kitchen waste. Among these isolates, the strain FH164 exhibited the highest stareh-hydrolyzing rate and LA production. 32.67g·L^-1 of LA was produced from 40.50g·L^-1 of soluble starch by strain FHI64 at pH 5.5 - 6.0 in 48h of fermentation. On the basis of its morphological and physio-biochemieal characteristics as well as 16S rDNA sequence, the strain FHI64 was tentatively identified as Streptococcus sp.. 28.23g·L^-1 of LA was obtained from kitchen waste by the strain FH164 employing an open fermentation mode（substrate non-autoclaved）, which was 19.2% higher than that of the fermentation without inoculum （control）.
出处
《环境科学》
EI
CAS
CSCD
北大核心
2006年第4期800-804,共5页
Environmental Science
基金
国家自然科学基金项目(50278024)
黑龙江省自然科学基金项目(E0316)
关键词
厨余垃圾
乳酸
解淀粉乳酸细菌
分离
发酵
kitchen waste
lactic acid
amylolytic lactic acid bacterla
isolation
fermentation
