摘要
目的探讨荧光定量聚合酶链反应(FQ-PCR)技术诊断结核病临床应用价值。方法应用FQ-PCR技术检测患者痰、外周血、体液(包括各种渗出液及脑脊液)中结核分枝杆菌DNA(TB-DNA),并与定性PCR、常规细菌学涂片法和培养法比较。结果112例结核病患者FQ-PCR、定性PCR、涂片法和培养法检测各类标本阳性率为59.82%,88.82%,25.89%,29.46%,非结核组43例FQ-PCR法阳性率是2.32%,PCR法是18.60%,FQ-PCR检测其标本TB-DNA的特异性为97.67%,敏感度为59.82%,阳性预测值为98.53%。结论FQ-PCR检测TB-DNA是一种快速简便、敏感性、特异性、可信性较高的方法,对诊断结核病具有临床意义,且适用于临床检验实验室,其含量变化对判断疗效有一定的提示作用。
Objective To evaluate the clinical value of fluorescence quantitative PCR（FQ-PCR） technique in diagnosing tuberculosis. Methods FQ-PCR was uesd to detect the tuberculosis DNA（TB-DNA） from 112 tuberculosis patients. The result was compared with qualitative PCR, routine bacteriological smear, and culture methods. Results The positive rates of FQ-PCR, qualitative PCR, routine hacterological smear, and culture were 59.8 %, 84.82 %, 25.89 %, and 29.4696, respectively. The FQ-PCR and PCR positive rates of 43 patients without tuberculosis were 2.32 % and 18.60 %. The FQ-PCR specificity and sensitivity were 97.53% and 59.82%, and its positive predictive vita was 98.53%. Conclusion FQ-PCR for detecting TB-DNA is rapid and simple, and has higher sensitivity, specificity and reliability, and is of clinic practical significance for diagnosing.
出处
《山西医科大学学报》
CAS
2006年第2期180-181,共2页
Journal of Shanxi Medical University
基金
原北京铁路局科研基金资助项目(京铁局技鉴字[2002]第09号
关键词
结核
诊断
聚合酶链反应
tuberculosis
diagnosis
polymers chain reaction
