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反复热性惊厥过程中γ-氨基丁酸B受体对一氧化氮/一氧化氮合酶体系的调节作用

Effect of gamma-aminobutyric acid B receptor on nitric oxide/nitric oxide synthase system during recurrent febrile seizures
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摘要 目的:探讨γ-氨基丁酸B受体(γ-aminobutyric acid B receptor,GABABR)对热性惊厥(febrile seizure,FS) 大鼠一氧化氮(nitric oxide,NO)/一氧化氮合酶(nitric oxide synthase,NOS)体系表达的影响。方法:将21 d龄SD 大鼠随机分为对照组、FS组、FS+巴氯芬(baclofen)组和FS+法克罗芬(phaclofen)组。采用热水浴诱导大鼠FS, 隔日诱导1次,共10次。采用分光光度计法测定大鼠血浆中NO含量;用原位杂交方法观察神经元型一氧化氮合酶(neuronal nitric oxide synthase,nNOS)mRNA表达情况;用免疫组化方法观察nNOS蛋白表达情况。结果:FS+ baclofen组NO含量低于FS组[(19.02±9.31)μmol/L比(40.03±9.12)μmol/L],同时nNOS蛋白和mRNA表达也较FS组减弱;而FS+phaclofen组NO含量高于FS组[(66.46±8.15)μmol/L比(40.03±9.12)μmol/L],同时nNOS蛋白和mRNA表达也较FS组增强。结论:反复热性惊厥过程中,GABABR的改变可影响NO/NOS体系的表达。 Objective:To explore the effect of γ-aminobutyric acid B receptor( GABABR) on nitric oxide ( NO)/nitric oxide synthase(NOS) system during recurrent febrile seizures (FS). Methods: Sprague-Dawley rats aged 21 days were randomly divided into four groups: control group, FS group, FS + baclofen group, FS+ phaclofen group. FS in rats were induced ten times in a bath of warm water, once every 2 days. The plasma level of NO was detected by the spectrophotometer; the expression of nNOS mRNA was examined by in situ hybridization; the expression of nNOS protein was observed by immunohistochemistry. Results:Compared with those in FS group, the plasma level of NO decreased [ (19.02 ±9.31 ) μmol/L vs (40.03 ±9. 12) μmol/L, and the expression of nNOS was down-regulated in FS ± baclofen group; the plasma level of NO increased [(66.46 ± 8. 15) μmol/L vs (40.03 ±9. 12) μmol/L and the expression of nNOS was up-regulated in FS + phaclofen group. Conlusionp GABABR modulated the expression of NO/NOS system during recurrent FS.
出处 《北京大学学报(医学版)》 CAS CSCD 北大核心 2006年第2期132-134,共3页 Journal of Peking University:Health Sciences
基金 卫生部临床学科重点项目(20010912)资助~~
关键词 惊厥 发热性 Γ-氨基丁酸 受体 GABA-A 一氧化氮 一氧化氮合酶 Seizures, febrile gamma-Aminobutyric acid Receptors, GABA-A Nitric oxide Nitric oxide synthase
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