摘要
目的:探讨Skp2的表达在非小细胞肺癌(nonsmallcelllungcancer,NSCLC)发生发展中的作用,及其与p27kip1和Ki67蛋白表达的关系。方法:应用免疫组化SP法检测Skp2、p27kip1和Ki67三种蛋白在60例NSCLC和20例正常支气管黏膜上皮组织中的表达。结果:NSCLC组织中Skp2蛋白表达的阳性率为48.33%(29/60),显著高于正常支气管黏膜上皮组织中的表达,P=0.000。Skp2的表达与肿瘤的组织学类型、肿瘤细胞的分化程度、TNM分期、淋巴结转移和患者吸烟与否显著相关,P值分别为0.038、0.005、0.019、0.010和0.002,但与患者的年龄及性别无关,P值分别为0.833和0.281。NSCLC组织中Skp2表达与p27kip1表达呈显著负相关,P=0.001;而与Ki67表达呈显著正相关,P=0.027。结论:Skp2在NSCLC组织中表达是上调的,可能是通过作用于细胞周期调控蛋白p27kip1,加速了对p27kip1泛素化依赖的蛋白降解,使其表达及代谢发生异常,导致细胞周期失控并促进细胞异常增殖,从而参与了NSCLC的发生和发展。
OBJECTIVE：To investigate the expression of Skp2 and its relationship with expressions of p27^kip1 and Ki-67 proteins in human non-small cell lung cancer（NSCLC）. METHODS： Immunohistochemical methods were applied to detect the expressions of Skp2, p27^kip1 and Ki-67 proteins in 60 surgical specimens from NSCLC patients and 20 normal bronchial epithelium. RESULTS： In NSCLC, the positive rate of Skp2 protein was 48. 33% （29/60）. Which was obviously higher than that in normal bronchial epithelium, P = 0. 000. There was no relationship between Skp2 expression and age （P = 0. 833） or sex （P=0.281）,respectively;otherwise, there was closely rdationship between Skp2 expression and histological subtype, cellular differentiation, TNM stage, lymph node metastasis, smoking, P= 0. 038, 0. 005, 0. 019, 0. 010 and 0. 002, respectively. Expression of Skp2 was negatively correlated with expression of p27^kip1 , P = 0. 001 ; but was positively correlated with expression of Ki-67, P = 0. 027. CONCLUSlONS： Expression of Skp2 is up-regulated significantly in NSCLC compared with normal bronchial epithelium, and overexpression of Skp2 reduces the protein level of p27^kip1 through ubiquitin-dependent degradation and causes disruption of cell cycle control and enhancement of the proliferative activity, indicating Skp2 playes an important role in oncogenesis and development of NSCLC.
出处
《中华肿瘤防治杂志》
CAS
2006年第2期93-96,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金资助项目(39870305)
