摘要
目的 分析不同临床病理因素对肝细胞癌患者血清蛋白质指纹图谱的影响。方法 收集112例肝细胞癌患者血清，采用弱阳离子交换蛋白质芯片为检测介质，经表面加强激光解吸电离-飞行时间-质谱测定得到蛋白质指纹图谱，分别以性别、甲胎蛋白、门静脉癌栓、肿瘤大小、肿瘤数目、肝硬化等病理特征分组，通过BioMarker Wizard Software比较各组间血清蛋白质组分的差异。结果 在质荷比为1100～30000范围内，112例肝细胞癌的血清蛋白指纹图谱中，共检测出100个有意义的蛋白峰。（1）比较肿瘤数目：单发与多发组（≥2），16个蛋白峰差异有统计学意义（P〈0．01）。（2）比较肿瘤最大直径：〉3cm与≤3cm组，仅1个蛋白峰差异有统计学意义；〉5cm与≤5cm组，4个蛋白峰差异有统计学意义；〉10cm与≤10cm组，3个蛋白峰差异有统计学意义（P〈0．01）。（3）比较门静脉肉眼癌栓组与无门静脉癌栓组：16个蛋白峰差异有统计学意义；比较门静脉镜下癌栓组与无门静脉癌栓组：仅2个蛋白峰差异有统计学意义；比较门静脉肉眼癌栓组与门静脉镜下癌栓组：8个蛋白峰差异有统计学意义（P〈0．01）。（4）按性别、肝硬化和甲胎蛋白分组比较，血清蛋白指纹图谱没有明显差异。结论 门静脉癌栓、肿瘤数目、肿瘤大小是影响肝细胞癌患者血清蛋白质指纹图谱的重要因素，而性别、肝硬化、甲胎蛋白对血清蛋白质指纹图谱没有明显影响；门静脉肉眼癌栓对血清蛋白质指纹图谱影响明显大于镜下癌栓；肿瘤大小以5cm分界对血清蛋白质指纹图谱影响最大。
Objective To investigate the effects of different clinicopathologic variables on serum protein fingerprint in hepatocellular carcinoma （ HCC ） patients. Methods Serum samples were collected from 112 HCC patients, Special serum protein or peptide spectra was determined by surface enhanced laser desorption/ionization-time of flight-mass spectrometry （SELDI-TOF-MS） measurement after treating the sample onto weak cation exchange （ WCX2 ） protein chip for each case. The serum protein profiles were compared by BioMarker Wizard Software among the patients stratified according to gender, AFP, presence of portal vein tumor thrombus（ PWIT）, tumor size, tumor number, presence of cirrhosis, respectively. Results According to serum protein fingerprints of 112 HCC patients, a total of 100 protein peaks were identified at the m/z value ranging from 1100 to 30 000. （1） Sixteen significant differential proteins were found between the groups of HCC with single tumor and those with multiple tumors （ P 〈 0. 01 ）. （2） Only one significant differential protein was found between the groups of HCC with tumor size 〉 3 cm and those with tumor size ≤3 cm, and 4 significant differential proteins between the groups of HCC with tumor size 〉5 cm and those with tumor size≤5 cm, while 3 significant differential proteins between the groups of HCC with tumor size 〉 10 cm and those with tumor size ≤ 10 cm （ P 〈 0. 01 ）. （ 3 ） Sixteen significant differential proteins were found between the groups of macroscopic portal vein tumor thrombus（Ma-PVTT） and those without PVTT（ N- PVTT） ; Only 2 significant differential protein were found between the groups of microscopic portal vein tumor thrombus （Mi-PVTT） and N-PVTT （P 〈 0. 01 ）; eight significant differential proteins were found between the groups of Ma-PVTT and Mi-PVTT （ P 〈 0. 01 ） . （ 4 ） No significant differential protein was found when patients stratified according to gender, presence of cirrhosis and AFP. Conclusions PVTT, tumor number and tumor size had significant effects on serum protein fingerprint, while no significant effect on serum protein from gender, presence of cirrhosis and AFP. The most profound impact on the serum protein was attained when cutoff was chosen to be presence of Ma-PVTT compared to less effect from Mi- PVTT and 5cm for tumor size.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2006年第7期445-449,共5页
Chinese Journal of Surgery
基金
"973"资助项目(2001CB510205)
"863"专项资助项目(2002BAC11A11)
上海市科研专项基金资助项目(03DJ14007)
关键词
癌
肝细胞
病理因素
蛋白质指纹图谱
Carcinoma , hepatocellular
Clinicopathologic variable
Protein fingerprint
