摘要
根据已知植物抗病基因的保守区域设计引物,从抗锈病小麦品种西农88基因组DNA扩增出3条与植物抗病基因同源的序列,分别为WRGA1、WRGA2和WRGA14。这三条同源片段均含有典型的NBS-LRR类抗病基因所拥有的保守性结构域Kinase-2a、Kinase-3a和疏水结构域(HD)．它们与部分已知NBS-LRR类抗病基因的氨基酸序列同源性为46．0%-9．9%,三个片段间在氨基酸水平上的同源性为80．7%-56．8%。Northern杂交表明WRGA1在小麦中受水杨酸正调控,属诱导型表达。
Specific primer sequences were designed on the basis of converse motifs of cloned resistance genes and subsequently used as PCR primers to amplify the resistance gene analog （RGA） in wheat （Triticum aestivum L.） Xinong 88 which possesses rust disease resistance. The amplified products of WRGA1,WRGA2 and WRGA14 were cloned and sequenced. The wheat RGAs contain some conserved motifs such as Kinase-2a,Kinase-3a and HD presenting in known NBS-LRR resistance genes from other plants and share between 46.0%-9.9% amino acid sequence identity with them. The amino acid sequence identity among three RGAs is 80.7%-56.8%. The WRGA1 expression was induced and detected by Northern blotting, which is positively regulated by salicylic acid.
出处
《分子细胞生物学报》
CSCD
北大核心
2006年第2期91-96,共6页
Journal of Molecular Cell Biology
基金
陕西省自然科学基金重点项目(2001SM24)
陕西省重点实验室科研计划项目(04JS07)
陕西省教育厅科学研究计划项目(05JK304)资助。
