摘要
人神经细胞SH-SY 5Y常规法培养至长满细胞瓶(120 cm2)后均分为A^I 9个小细胞瓶(30 cm2),37℃培养15 h后,B^D组在42℃条件下分别进行0.5、1和2 h的持续热应激处理,而E^I组于42℃热应激处理0.5 h后,37℃分别恢复1、3、8、12、24 h。持续热应激阶段,朊蛋白(P rP)和热休克蛋白(HSP)90 mRNA水平显著提高(P<0.01),应激2 h时,P rP和HSP 90 mRNA水平分别约为对照组(A组)的2.5和5倍;在应激后恢复初期(1~3 h),P rP和HSP 90mRNA水平也显著升高(P<0.01),其中恢复到3 h时,P rP和HSP 90 mRNA水平分别为对照组的9和7倍;当恢复到12~24 h时,HSP 90和P rP mRNA的转录水平与对照组差异不显著。结果表明热应激可以提高HSP 90和P rP mRNA的转录水平。应激时P rP mRNA转录水平的提高暗示着P rP在细胞应激中起着一定的生物作用。
Cells were maintained at 37℃ in a humidified 50% CO2/air incubator. Once the cells became confluent in the large flask （120 cm^2 in size）, they were split equally and distributed into nine （A-I） smaller flasks（30 cm^2 in size）. The cells in the smaller flasks were kept at 37 ℃ in the incubator for 15 h prior to heat-shock treatment, at each time, B-D flasks were removed to 42℃ incubator for 0. 5, 1, 2 h and E-I were recovered into the incubator at 37℃ for 1, 3, 8, 12, 24 h subsequent to heat-shock treatment at 42℃ for 0.5 h. There was an about 2.5 and 5-fold increase in PrP and HSP90 mRNA levels 2 h following heat shock at 42 ℃. In addition, a 9 and 7-fold increase in mRNA level of PrP and HsP90 was found following heat shock 0.5 h at 42 ℃ and recovery for 3 h at 37 ℃ and there was no significant difference with control following heat shock 0.5 h at 42 ℃ and recovery for 12 h and 24 h at 37 ℃. These results suggested that heat stress induced the elevation of both PrP and HSP90 mRNA. The up-regulation of prion-protein mRNA implies that PrP may play a role in cellular stress.
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2006年第1期14-18,共5页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金资助项目(39770569
30170682)
中德农业科技合作项目
