摘要
目的：建立硅胶管采集工作场所空气中硝基苯的液相色谱分析方法。方法：硅胶管吸附空气中硝基苯，样品经甲醇解吸，HPLC检测。结果：当空气中硝基苯浓度范围在0．67～6．67mg／m^3（参照国标PC-TWA2mg／m^3）时，方法的变异系数为0．3％，回归方程式Y=42769X＋74．5，相关系数r=0．9999，检出限0．03μg／ml，以采集3L空气样品计，最低检出浓度0．02mg／m^3。平均解吸效率为98．6％，硅胶吸附8．00μg硝基苯，样品放置2周，回收率仍可达98．8％，变异系数为0．6％。在本实验条件下与苯胺类化合物有较好的分离。结论：本法可应用于工作场所空气中硝基苯浓度的检测。
Objective：To establish a liquid chromatographic （LC） analytical method for nitrobenzene in the workplace using silica gel tube for sampling. Methods：Silica gel was used to absorb nitrobenzene. Sample was desorpted by carbinol and analyzed by HPLC. Results：The coefficient of variation for the whole analytical method was in the range of 0. 67 ～6. 67 mg/m^3 and the variant coefficient was 0. 3%. Regression formula was Y = 42769X ＋ 74. 5, r = 0. 9999. Determination limit was 0. 03 μg/ml. The lowest determinable concentration was 0. 02 mg/m^3 when 3 liter workplace air sample was collected. Desorption efficiency was 98. 6%. After taking 8. 00 μg of nitrobenzene by silica gel tube and two weeks storage, its recovery efficiency was 98. 8%. The coefficient of variation was 0. 6% . It had sound separation with phenyl amines compound under this experimental condition. Concluslon：This method can be used to determine the concentration of nitrobenzene in the workplace air.
出处
《中国卫生检验杂志》
CAS
2006年第4期391-393,共3页
Chinese Journal of Health Laboratory Technology
关键词
硝基苯
硅胶管
溶剂解吸
液相色谱
Nitrobenzene
Silica gel tube
Solvent desorption
Liquid chromatograph
