摘要
目的：建立高效液相色谱-电化学检测法（HPLC-ECD）测定人尿中8-羟基脱氧鸟嘌呤核苷（8-OHdG）的检测方法。方法：采用Bond Elute C18小柱固相萃取尿液，ODS C18分析柱，8％甲醇醋酸缓冲液为流动相，电化学检测器检测，电极电压750mV。结果：本方法线性范围25～400μg/L，r=0．9892，回收率为91．2％～108．8％，批内和批间精密度分别为3．9％～7．5％和4．3％～8．6％，检测限为2μg/L。结论：本方法操作简便、准确度较好，已应用于正己烷接触者现场尿样的测定。
Objective： To establish a HPLC -ECD method to measure 8- oxo- deoxyguanosine （8 -OHdG） in human urine. Methods：The urinary sample was pretreated with a solid phase extraction （SPE） column. Then the chromatography was performed by an ODS Cls column with ECD detector and mobile phase of acetic acid buffer which contained 8% methanol. Results：The linear range was 25 400 μg/L, r =0. 9892, the recovery rate was 91.2% ～ 108.8%, the intra - and inter - day precisions were 3.9% ～7.5% and 4. 3% ～ 8. 6% respectively, the detection limit was 2 μg/L. Conclusion： It is a better method for the determination of 8 - OHdG in human urine.
出处
《中国卫生检验杂志》
CAS
2006年第4期405-406,共2页
Chinese Journal of Health Laboratory Technology
