摘要
目的：建立测定全血中秋水仙碱的简便、灵敏、准确的高效液相色谱二极管阵列（DAD）检测方法。方法：全血样品经1％三氟醋酸乙腈溶液提取后，用Waters Oasis MCX小柱进行净化，在ZORBAX Eclipse XDB-C8柱（150mm×2．1mm×5μm）上以乙腈＋0．04％三氟醋酸（23＋77）为流动相，二极管阵列（DAD）紫外245nm检测，柱温35℃，外标法定量。结果：秋水仙碱在0．50-50．0mg／L范围内均具有良好的线性，血样中定量检出下限为0．10mg／L，方法回收率为88．1％-101．9％，RSD小于10％。结论：该方法的建立具有灵敏度高、操作简便、快速、准确，适用于中毒病人的中毒诊断。
Objective：To establish a simple,sensitive and accurate method for the determination of colchicine in whole blood by HPLC - DAD. Methods： After colchicine in whole blood was extracted by 1% Trifluoroacetic - Acetonitrile, the sample was cleaned with solid phase extraction using Oasis MCX cartridges and the colchicine was separated on ZORBAX Eclipse XDBC8 column（ 150 mm × 2. 1 mm ×5 μm） with DAD detection at 245 nm by using the mobile phase consisting of Acetonitrile （23%） and 0. 04% Trifluoroacetic （77%）. The column temperature was controlled at 35℃. Results：Calibration curve was linear within the range of 0. 50 - 50. 0 mg/L and the limit of quantification was found to be 0. 10 mg/L The extraction recovery was between 88.1% - 101.9% ,the RSD was less than 10%. Conclusion：This method is sensitive, fast and accurate for determination of trace colchicine in whole blood and can be used in the diagnosis of the clinical poisoned patients.
出处
《中国卫生检验杂志》
CAS
2006年第4期418-419,494,共3页
Chinese Journal of Health Laboratory Technology
