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应用基因芯片技术筛查人子宫内膜容受性相关基因 被引量：1

The study on gene expression profiles in human endometrial receptivity by DNA microarray technique
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摘要 目的探讨人子宫内膜容受性相关基因的差异表达。方法应用含14000条基因的cDNA表达谱基因芯片分析分泌早期与分泌中期子宫内膜基因的差异表达。结果所检测的14000个基因中,分泌早期子宫内膜与分泌中期子宫内膜之间存在显著差异表达基因313个。其中,分泌中期子宫内膜表达上调基因数为175个,下调基因数为138个。结论子宫内膜容受性的建立受许多因素的调控,应用基因芯片技术可以快速、高通量的筛查出相关基因,从而有可能找到合适的分子标志物作为子宫内膜容受性的临床诊断指标。 Objective： To explore the gene expression profiles of the receptive endometrium. Methods： The differentially expressed genes were analyzed between the early secretory （ day LH ＋ 2） endometrium and the mid - secretory （ day LH ＋ 7 ） endometrium, using cDNA chip containing 14000 genes, Results： There were total 313 genes significantly differently expressed in the mid - secretory endometrium compared with the early secretory endometrium. Among them, 175 genes were upregulated and 138 were down- regulated. Conclusion： Many factors have effect on human endometrial receptivity, cDNA microarray technique is effective in screening out the differentially expressed genes between 2 different tissues, Thus, we may find proper molecular markers as the diagnosis criteria of the endometrial receptivity.
作者 章汉旺 范莉 赖巧红
机构地区 华中科技大学同济医学院附属同济医院妇产科
出处 《中国优生与遗传杂志》 2006年第4期18-20,共3页 Chinese Journal of Birth Health & Heredity
基金 湖北省科技攻关项目(2004AA301C38)
关键词 子宫内膜 基因表达谱 接受性 着床 Endometrium Gene expression profiles Receptivity Implantation
分类号 R450 [医药卫生—治疗学]
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参考文献13

  • 1Medlin JF.Timely toxicology[J].Environ Health Perspect,1999,107(5):A256-258.
  • 2Amundson SA,Bittner M,Chcn Y,et al.Fluorescent eDNA microarray hybridization rcveals complexity and heterogeneity fo cellular genotoxic stress respinse[J].Oncogene,1999,18(24):4759-4760.
  • 3Afshari CA,Nuwaysir EF.Barrett JC,Application of complementary DNA microarray technology to carcinogen identification toxicology and drug safety evaluation[J].Cancer Res,1999,59(19):4759-4760.
  • 4White CA,Salamonson LA.A guide to issues in microarray analysis:application to endometrial biolog[J].Rproduction,2005 Jul,130(1):1-13.
  • 5Johannisson E,Morphological and histocheical factors related to implantation[J].Bailliere's Clin Obstet Gynecol,1991,5:191-209.
  • 6Katahira J,Sugiyama H,Inoue N,et al.Clostridium perfringens entertoxin utilizes two structurally related membrane proteins as functional receptors in vivo[J].Biol Chem,1997:26652-26658.
  • 7Michl P,Buchholz M,Rolke M,et al.Chaudin-4:a new target for pancreatic cancer treatment using Clostridium perfringens enterotoxn[J].Gastroenterology,2001,121:678-684.
  • 8Hough CD,Sherman-Baust CA,Pizer ES,et al.Large-scale serial analysis of gene expression reveals genes differentially expresscd in ovarian cancer[J].Cancer Res,2000,60:6281-6287.
  • 9Van Itallie C,Rahner C,Anderson JM,Regulated expression of claudin-4 decreases paracellular conductance through a selective decrease in sodium permeability[J].J Clin Invest,2001,107:1319-1327.
  • 10Heiskala M,Peterson PA,Yang Y,The roles of claudin superfamily proteins in paracellular transport[J].Traffic,2:93-98.

同被引文献14

  • 1Cavagna M, Mantese JC. Biomarkers of endometrial receptivity - a review. Biol Reprod, 2004, 70 (3): 729
  • 2Kao LC, Tulac S, Lobo S et al. Global gene profiling in human endo- metrium during the window of implantation. Endocrinology, 2002, 143 (6) : 2119 -38
  • 3Riesewijk A, Matin J, Van R et al. Gene expression profiling of human endometrial receptivity on days LH + 2 versus LH + 7 by mircoarcay technology. Mol Hum Reprod, 2003, 9 (5): 253-64
  • 4Carson D, Lagow E, Thathiah Aet al. Changes in gene expression during the early to midluteal (receptive phase) transition in human endometrium detected by high - densitymicroarray screening. Mol Hum Repred, 2002, 8 (9): 871-9
  • 5Pfaffl MW, Horgan GW, Dempfle L. Relative expression software tool (REST) for group - wise comparison and statistical analysis of relative expression results in real -time PCR. Nucleic Acids Res, 2002, 30 (9) : 36
  • 6Kyle J, Rachana A, Patrice J .et al. The claudin gent family : expression in normal and neoplastic tissues. BMC Cancer, 2006, 6 (12) : 186
  • 7Michl P, Buchholz M, Rolke M et al. Chaudin - 4: a new target for pancreatic cancer treatment using Clostridium perfringens enterotoxn. Gastroenterology, 2001, 121 (3) : 678 -84
  • 8Anderson JM, Van Itallie CM. Tight junctions and the molecular basis for regulation of paracellular permeability. Am J Physiol, 1995, 269 (4) : 467
  • 9Go M, Kojima T, Takano K et al. Expression and function of tight junctions in the crypt epithelium of human palatine tonsils. J Histochem Cytochem, 2004, 52 (12): 1627-38
  • 10Bergh PA , Navot D. The impact of embryonic development and endo- metrial maturity on the timing of implantation. Fertil Steril, 1992, 58 (3) : 537 -42

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中国优生与遗传杂志
2006年 第4期

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