摘要
目的探讨芪丹通脉片及其拆方对脑缺血再灌注损伤神经细胞凋亡及Bcl-2蛋白表达的影响,并研究方剂配伍的意义。方法用线栓法制备大鼠局灶性脑缺血再灌注模型,分为假手术组、模型组、芪丹通脉片总方组与黄芪组、活血组。末端脱氧核糖核酸转移酶介导的原位末端标记法(TUNEL法)检测各组大鼠脑组织神经细胞凋亡,免疫组织化学法检测Bcl-2蛋白的表达。结果与假手术组比较,模型组凋亡神经细胞及Bcl-2蛋白阳性细胞表达增高(P<0.01)。与模型组比较,芪丹通脉片总方组与黄芪组、活血组凋亡神经细胞表达降低,Bcl-2蛋白阳性细胞表达增多(P<0.01),且与拆方组比较,总方组的作用最强(P<0.01)。结论芪丹通脉片及其拆方可能通过上调Bcl-2蛋白的表达抑制神经细胞凋亡,对脑缺血再灌注损伤有明显的保护作用。芪丹通脉片总方组的作用明显优于黄芪组、活血组。
Objective To investigate the effects of Qi Dan Tong Mai Tablets （QDTMT） and its separate components on apoptosis of neuronal cells and expression of Bcl-2 protein after cerebral ischemia and reperfusion, and to study the compatibility of the prescription. Methods The string inserting method was employed to make the model of focal cerebral ischemia and reperfusion. Rats were divided into sham-operated group, model group, Astragalus group, blood-activating group and QDTMT group. Neuronal apoptosis in rats＇ brain tissue of each group was analyzed by TdT-mediated dUTP nick end labeling （TUNEL）. Expression of Bcl-2 protein was mea- sured by immuno-histochemical method. Results Compared with those in sham-operated group, the apoptosis of neuronal cells and expression of Bcl-2 protein increased in model group（ P 〈 0.01）. Compared with those in model group, the apoptosis of neuronal ceils in Astragalus group, blood-activating group and QDTMT group decreased,while the expression of Bcl-2 protein increased（ P 〈 0.01）, and QDTMT group had the strongest effects compared with separate components groups（P 〈 0.01 ）. Conclusions QDTMT and its separate components have significant protective effect which might result from up-regulating the expression of Bcl-2 protein and inhibiting neuronal cell apoptosis. Neuroprotective effect of QDTMT is better than that of Astragalus and bloodactivation.
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2006年第2期133-135,共3页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
全军新药开发课题(2001)
关键词
脑缺血
再灌注损伤
细胞凋亡
中药配伍
brain ischemia
reperfusion injury
apoptosis
compatibility
