内源性氢化可的松为探针的体内CYP3A活性测定被引量：3

Determination of CYP3A activity in vivo by the ratio of urinary endogenous 6β-hydroxycortisol to cortisol

下载PDF

导出

摘要目的建立测定精神病患者体内CYP3A酶活性的方法。方法直接收集20例精神病住院患者晨尿液,测定尿液中内源性氢化可的松与6β-羟基氢化可的松的含量,以6β-羟基氢化可的松/氢化可的松来评估CYP3A酶的活性。结果患者尿液中6β-羟基氢化可的松与氢化可的松的比值为(8.12±4.36)。结论CYP3A酶的活性可以通过测定6β-羟基氢化可的松与氢化可的松的比值进行预测。 OBJECTIVE To establish a method for the determination of cytochrome P450 3A activity in vivo. METHODS The urine of 20 schizophrenia patients was collected in the morning. The endogenous 6β-hydroxycortisol and cortisol concentrations in the urine were determined. The ratio of 6β-hydroxycortisol to cortisol was demonstrated to assess the CYP3A activity in vivo. RESULTS The ratio of 6β-hydroxycortisol to cortisol was 8. 12-1-4.36. CONCLUSIONS The CYP3A activity in vivo can be predicted by the ratio of urinary endogenous 6β-hydroxycortisol to cortisol.

作者尧臣彬李坤艳李焕德

机构地区湖南省人民医院药剂科湖南省肿瘤医院药剂科临床药学室中南大学湘雅二医院临床药学室

出处《中南药学》 CAS 2006年第2期104-106,共3页 Central South Pharmacy

关键词 6β-羟基氢化可的松氢化可的松 CYP3A 高效液相色谱法 6β-hydroxycortisol cortisol CYP3A HPLC

分类号 R968 [医药卫生—药理学]

引文网络
相关文献

参考文献8

1[1]Mathijssen RH,de Jong FA,van Schaik RH,et al.Prediction of irinotecan pharmacokinetics by use of cytochrome P450 3A4 phenotyping probes[J].J Natl Cancer Inst,2004,96(21):1585-1592.
2[2]Kinirons MT,Krivoruk Y,Wilkinson GR,et al.Effects of ketoconazole on the erythromycin breath test and the dapsone recovery ratio[J].Br J Clin Pharmacol,1999,47(2):223-225.
3[3]Tateishi T,Watanabe M,Nakura H,et al.CYP3A activity in European American and Japanese men using midazolam as an in vivo probe[J].Clin Pharmacol Ther,2001,69(5):333-339.
4[4]Hu Z,Gong Q,Hu X,et al.Simultaneous determination of 6beta-hydroxycortisol and cortisol in human urine and plasma by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity[J].J Chromatogr B Analyt Technol Biomed Life Sci,2005,826(1-2):238-243.
5[5]Zhiri A,Mayer HA,Michaux V,et al.6 beta-hydroxycortisol in serum and urine as determined by enzyme immunoassay on microtitre plates[J].Clin Chem,1986,32(11):2094-2097.
6[6]El Desoky ES,Mohamed HO,Farghaly WM,et al.Study of urinary 6 beta-hydroxycortisol/cortisol ratio in spot urine sample as a biomarker of 3A4 enzyme activity in healthy and epileptic subjects of Egyptian population[J].Pharmacol Res,2005,51(6):575-580.
7[7]Rouits E,Boisdron-Celle M,Morel A,et al.Simple and sensitive high-performance liquid chromatography method for simultaneous determination of urinary free cortisol and 6beta-hydroxycortisol in routine practice.For CYP 3A4 activity evaluation in basal conditions and after grapefruit juice intake[J].J Chromatogr B Analyt Technol Biomed Life Sci,2003,793(2):357-366.
8[8]Furuta T,Mori C,Suzuki A,et al.Simultaneous determination of 6beta-hydroxycortisol and cortisol in human urine by liquid chromatography with ultraviolet absorbance detection for phenotyping the CYP3A activity determined by the cortisol 6beta-hydroxylation clearance[J].J Chromatogr B Analyt Technol Biomed Life Sci,2004,801(2):165-171.