摘要
利用金属钛离子注入天冬氨酸转氨酶生产菌株U12．3-19进行诱变,摸索金属离子与该生产菌株的互相作用规律,统计变异情况,并与氮离子注入诱变和紫外诱变的效果做了比较,得到了较高的突变率和较广的突变谱。通过大量筛选,在苯丙酮酸的浓度为20g/L的条件下,得到了使L-苯丙氨酸产量提高22．67％的高产菌株U12-25-116-J75。
Mutagenic effects of the transaminase producing bacterium Escherichia coli U12-3-19 by low energy metal ion （Ti^2＋） implantation were investigated. And the different mutagenic effects of E.coli irradiated with UV and low-energy ions were compared. The results showed that the E.coli after the ion implantation could achieve higher rate of mutation and wide mutational spectrum. A high yield L-phe strain has been bred from this. In the shaking flask, the L-phe productivity of strain U12-25-116-J75 increased by 22.67% than of the comparison strain with the PPA concentration of 20g/L.
出处
《辐射研究与辐射工艺学报》
EI
CAS
CSCD
北大核心
2006年第2期125-128,共4页
Journal of Radiation Research and Radiation Processing
