摘要
目的探索葡聚糖硫酸钠(dextransulfatesodium,DSS)诱导UC急性模型的浓度及时间。方法用C57BL/6小鼠自由饮用DSS溶液(3%、5%、8%)的方法建立急性UC模型,正常组饮用蒸馏水作为对照。通过疾病活动指数(DAI)和肠黏膜组织损伤指数(HI)来评价结肠的形态和组织病理学改变。结果DAI评分随着浓度(0、3%、5%和8%)的增加呈线性增加(1.50±0.53、7.71±1.38、9.14±1.03和9.71±0.61),呈浓度依赖性(组间比较P<0.05);远端结肠HI评分(0、5.93±1.65、7.00±1.07、7.75±0.46)和近端结肠HI评分(0、4.25±1.58、5.13±3.17、6.50±1.41)无浓度依赖性(仅3%组vs8%组P<0.05);死亡率随浓度的增加而升高(0、7.10%、28.60%和57.10%)。结论DSS模型的临床表现和病理表现与人类UC及其相似,是理想的UC模型。DSS模型结肠炎症程度的轻重与浓度无明显相关。采用C57BL/6小鼠饮用3%DSS(分子量为40000左右)溶液5天的方法可建立理想的UC急性模型。
Objective To explore the concentration and time of dextran sulfate sodium （DSS） to induce mice acute ulcerative coutis model. Methods The C57BL/6 mice were fed with 3% ,5% and 8% DSS solution to induce acute colitis, the control group were only fed with distilled water. In all groups, the macroscopic and histopathological damages of colon were evaluated by assessing disease active index （DAI） and histological index （HI）. Results A linear increase in DAI （ 1.50 ± 0.53,7.71 ± 1.38,9.14 ± 1.03 and 9.71 ± 0.61, respectively） was noted with increasing concentrations. The HI of distal colon （0,5.93 ± 1.65,7.00 ± 1.07,7.75 ±0.46） and proximal colon（0,4.25 ± 1.58,5.13 ± 3.17,6.50 ± 1.41） did not correlate with DSS concentration （only P 〈 0.05 between 3 % and 8 % group）. An increase of mortality rote was noted with increased DSS concentration （0,7.10 %, 28.60 % and 57.10 % ）. Conclusion The clinical and histological features of DSS-inducod acute colitis ressemble with UC in human. C57BL/6 mice feed with 3% DSS （molecular weight is about 40 000） solution for five days may establish an ideal acute UC model.
出处
《胃肠病学和肝病学杂志》
CAS
2006年第2期130-133,共4页
Chinese Journal of Gastroenterology and Hepatology
