期刊文献+

小鼠葡聚糖硫酸钠急性溃疡性结肠炎模型的建立和评价 被引量:28

The establishment of acute ulcerative colitis in mice induced with DSS
下载PDF
导出
摘要 目的探索葡聚糖硫酸钠(dextransulfatesodium,DSS)诱导UC急性模型的浓度及时间。方法用C57BL/6小鼠自由饮用DSS溶液(3%、5%、8%)的方法建立急性UC模型,正常组饮用蒸馏水作为对照。通过疾病活动指数(DAI)和肠黏膜组织损伤指数(HI)来评价结肠的形态和组织病理学改变。结果DAI评分随着浓度(0、3%、5%和8%)的增加呈线性增加(1.50±0.53、7.71±1.38、9.14±1.03和9.71±0.61),呈浓度依赖性(组间比较P<0.05);远端结肠HI评分(0、5.93±1.65、7.00±1.07、7.75±0.46)和近端结肠HI评分(0、4.25±1.58、5.13±3.17、6.50±1.41)无浓度依赖性(仅3%组vs8%组P<0.05);死亡率随浓度的增加而升高(0、7.10%、28.60%和57.10%)。结论DSS模型的临床表现和病理表现与人类UC及其相似,是理想的UC模型。DSS模型结肠炎症程度的轻重与浓度无明显相关。采用C57BL/6小鼠饮用3%DSS(分子量为40000左右)溶液5天的方法可建立理想的UC急性模型。 Objective To explore the concentration and time of dextran sulfate sodium (DSS) to induce mice acute ulcerative coutis model. Methods The C57BL/6 mice were fed with 3% ,5% and 8% DSS solution to induce acute colitis, the control group were only fed with distilled water. In all groups, the macroscopic and histopathological damages of colon were evaluated by assessing disease active index (DAI) and histological index (HI). Results A linear increase in DAI ( 1.50 ± 0.53,7.71 ± 1.38,9.14 ± 1.03 and 9.71 ± 0.61, respectively) was noted with increasing concentrations. The HI of distal colon (0,5.93 ± 1.65,7.00 ± 1.07,7.75 ±0.46) and proximal colon(0,4.25 ± 1.58,5.13 ± 3.17,6.50 ± 1.41) did not correlate with DSS concentration (only P 〈 0.05 between 3 % and 8 % group). An increase of mortality rote was noted with increased DSS concentration (0,7.10 %, 28.60 % and 57.10 % ). Conclusion The clinical and histological features of DSS-inducod acute colitis ressemble with UC in human. C57BL/6 mice feed with 3% DSS (molecular weight is about 40 000) solution for five days may establish an ideal acute UC model.
出处 《胃肠病学和肝病学杂志》 CAS 2006年第2期130-133,共4页 Chinese Journal of Gastroenterology and Hepatology
关键词 溃疡性结肠炎 葡聚糖硫酸钠 C57BL/6小鼠 浓度 Ulceative colitis (UC) Dextran sulfate sodium (DSS) C57BL/6 mice Concentration
  • 相关文献

参考文献19

  • 1Egger B, Bajai-Elliott M, MacDonald TT, et al. Characterisation of acute murine dextran sodium sulphate colitis:cytokine profile and dose dependency. Digestion, 2000,62(4):240-248.
  • 2吴小平,凌奇荷.实验性溃疡性结肠炎模型的研究[J].湖南医科大学学报,1998,23(4):359-360. 被引量:18
  • 3Murano M, Maemura K, Hirata I, et al. Therapeutic effect of intracolonically administered nuclear factor kappa B (p65) anti-sense oligonucleotide on mouse dextran sulphate sodium (DSS)-induced colitis. Clin Exp Immunol,2000,120(1):51-58.
  • 4Dieleman LA,Palmen MJ,Akol H,et al. Chronic experimental colitis induced by dextran sulphate sodium (DSS) is characterized by Th1 and Th2 cytokines. Clin Exp Immunol, 1998,114(3) :385-391.
  • 5江学良 权启镇 等.鱼腥草治疗溃疡性结肠炎大鼠对结肠压力的影响[J].世界华人消化杂志,1999,7:639-639.
  • 6Papanicolaou DA, Wilder RL, Manolagas SC, et al. The pathophysiologic roles of interleukin-6 in humans isease. Am Intern Med, 1998,128(2) :127-137.
  • 7台卫平,罗和生.大鼠离体结肠炎模型的制作[J].中国病理生理杂志,2002,18(2):222-224. 被引量:9
  • 8Tautog JD, Breban M, Simmons WA, et al. New experimental model of 1BD:HLA-B27 transgenic. Res Cli Foru, 1993,15(2) : 173.
  • 9Aiko S, Grisham MB. Spontaneous intestinal inflammation and nitric oxide metabolism in HLA-B27 transgenic rats. Gastroenterology, 1995,109(1):142-150.
  • 10李军华,于皆平,何小飞,徐细明.核因子-κB在大鼠实验性溃疡性结肠炎肠组织的表达及其意义[J].世界华人消化杂志,2003,11(2):214-218. 被引量:24

二级参考文献13

共引文献57

同被引文献291

引证文献28

二级引证文献168

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部