摘要
目的研究几种常用干细胞标记物在人胃肠道黏膜干细胞中的表达。方法采用免疫组织化学SP法对5例正常胃黏膜、5例正常小肠黏膜及5例正常降结肠黏膜中Musashi1、端粒酶逆转录酶(TERT)、Oct4和Nestin的表达情况进行研究。结果胃黏膜中Musashi1和TERT阳性细胞主要位于腺体峡部,Oct4阳性细胞多位于腺体基底部,Nestin阳性细胞主要分布在胃黏膜间质以及腺体颈部。小肠黏膜中Musashi1和TERT阳性细胞主要在距隐窝底部4～7个细胞的位置,Oct4阳性细胞散在分布于腺体内,Nestin阳性细胞均分布在小肠黏膜间质,无腺体内着色。降结肠黏膜中Musashi1和TERT阳性细胞分别主要在距隐窝底部1～4个和1～5个细胞的位置,Oct4阳性细胞散在分布在腺体内和黏膜间质,Nestin阳性细胞分布黏膜间质内,腺体内无表达。结论Musashi1和TERT的表达与胃肠道干细胞的位置较吻合,可认为是胃肠道相对特异的干细胞标记物。而Oct4与Nestin做为胚胎干细胞和神经干细胞的标记物被广泛应用,但不能用于胃肠道干细胞的标记。
Objective To study the expression of four general stem cell markers in the gastrointestinal mucosa for identification of the gastrointestinal stem cells. Methods SP immunohistochemical technique was used to analyze the expres sion of Musashi-1 ,TERT,Oct-4 and Nestin in 5 cases of normal human gastric, small intestinal and descending colonic mucosa, respectively.Results In gastric mucosa, the location of Musashi-1-positive cells and TERT-positive cells was principal in the isthmus region of the gastric glands. The majority of Oct-4-positive cells were situated in the basal region. Nestin-positive cells were mainly in the gastric mesenchyma and the neck region of the glands. In mucosa of small intestine, Musashi-1- positive cells and TERT-positive cells were mainly located between cell positions 4 and 7. Oct-4-positive cells were diffusely distributed in the crypts. Nestin-positive cells were in the mesenchyma of small intestine, none of which in the crypts. In mucosa of descending colon, Musashi-1-positive cells were mainly located between cell positions 1 and 4, and TERT-positive cells 1 and 5. Oct-4-positive cells were diffusely distributed in the crypts and the mesenchyma. Nestin-positive cells were all in the mesenchyma, none in the crypts. Conclusion The distribution of Musashi-1-positive cells and TERT-positive cells corresponds with that of stem cells, implying that they can be putative gastrointestinal stem cell markers. Though Oct-4 and Nestin are widely used as the embryonic and nerve stem cell markers, they can not be utilized in marking the gastrointestinal stem cells.
出处
《胃肠病学和肝病学杂志》
CAS
2006年第2期199-202,共4页
Chinese Journal of Gastroenterology and Hepatology
