摘要
目的探讨IL-4和IL-17在实验性变应性鼻炎(AR)发病机制中的作用及地塞米松(DEX)对实验动物血清IgE、IL-4和IL-17水平的影响及其意义。方法用卵清蛋白(OV)建立AR大鼠模型,给予口服DEX两周后,用被动皮肤过敏原试验(PCA)检验大鼠模型制备及药物治疗后情况,并用酶联免疫吸附试验法(ELISA法)对各组大鼠外周血清IL-4、IL-17和IgE的变化进行观察。结果AR组IgE(38.67±4.13Pg/ml)、IL-4(106.31±12.9Pg/ml)和IL-17(30.61±2.55Pg/ml)水平显著高于正常组(分别是23.27±1.36Pg/ml、93.65±7.78Pg/ml和22.56±2.67Pg/ml)(P<0.05),IL-4、IL-17水平与IgE水平正相关;口服DEX两周后,实验大鼠外周血IgE(24.23±4.38Pg/ml)、IL-4(92.67±16.4Pg/ml)和IL-17(22.97±2.50Pg/ml)水平分别较AR模型组显著下降(P<0.05)。结论IL-4和IL-17在AR中有重要的调节作用,是IgE的重要影响因素,DEX降低IgE可能是通过影响IL-4和IL-17水平而实现的。
Objective To investigate the effects of dexamethasone（DEX） on IgE, IL-4 and IL- 17 levels in the serum of rats with experimental allergic rhinitis（EAR） and the implications of IL-4 and IL-17 in the pathogenesis of allergic rhinitis. Methods EAR model was induced by the method of using ovalbumin（OV） in rats at first. Then, they were divided into treating group（TG） and modeling group（MG） respectively, with another healthy controlling group（HCG） of rats set up at the same time. DEX was orally giwen to the animals of TG for 2 weeks. Passive cutaneous antigen assay （PCA） was utilized to evaluate the conditions of EAR and enzyme linked immunosorbent assay （ELISA） was taken to detect the serum levels of IgE, IL-4 and IL-17 in the experimental rats. Resuits The serum levels of IgE, IL-4 and IL-17 were 38.67±4.13 Pg/ml, 106.31±12.9 Pg/ml and 30.61±2.55 Pg/ml respectively among the animals of MG, significantly higher than the values of 23.27±1.36 Pg/ml, 93.65±7.78 Pg/ml and 22.56±2.67 Pg/ml respectively in HCG（P 〈 0.05） andwith significant positive correlations among the levels of IgE to IL-4 and IL-17. With 2 weeks of DEX orally taking, these values of IgE（24.23 ±4.38 Pg/ml）, IL-4（92.67±16.4 Pg/ml） and IL-17 （22.97±2.50 Pg/ml） were significantly decreased respectively in TG, with significant differences when compared with those of MG（P〈0.05）.Conelusions IL-4 and IL-17 may hold regulatory effects on the pathogenesis of AR and are the important affecting factors on the level of IgE. The pharmacological effect of DEX on IgE to reduce its serum content may be brought about via the effect on the serum levels of IL-4 and IL-17.
出处
《中国中西医结合耳鼻咽喉科杂志》
2006年第2期69-72,T0001,共5页
Chinese Journal of Otorhinolaryngology in Integrative Medicine
基金
上海市跨世纪优秀学科带头人"百人计划"项目资助(BR003)
