摘要
目的定量分析P物质(substanceP,SP)作用时间对人增生性瘢痕(hypertrophicscar,HS)中肥大细胞(mastcell,MC)组织胺释放的影响,探讨HS中二者的相互作用及作用的时间条件。方法人HS活体组织块切下后立即处理,修剪成0.5～1mm3大小,测定在相同浓度的SP、不同的SP作用时间下,MC的脱颗粒情况。荧光法测定作用后上清液中组胺含量,计算组胺释放率。结果在HS中,SP以时间依赖方式刺激MC组胺的释放,且HS中SP对MC作用强于正常皮肤。结论HS中的SP与MC的关系非常密切,存在作用时间依赖性,有利于研究SP作用与HS的相互作用及作用条件。
Objective To detect a quantitative relation between reaction time of substance P （SP） and histamine release of mast cells （MC） in hypertrophic scar （HS） and discuss the interaction and time-effect of substance P and histamine release in HS. Methods The HS specimens were from the 4 to 7-year-old scalded or burnt patients who underwent operation because of cicatricial contraction. The normal skins were the autograft. HS specimens were cut into 0.5 - 1 mm3 , then treated with 5×10^-5moL/L SP for 5, 15, 30, 60 min. The degranulation of MC was detected and the histamine released by mast cells in the supernate after SP treatment was examined by immunofluorescence. Conclusion SP and MC are of a close relationship in HS, and SP can affect histamine release of mast cell in time-dependent manner.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第8期841-843,共3页
Journal of Third Military Medical University
关键词
增生性瘢痕
P物质
肥大细胞
组胺释放
hypertrophic scar
substance P
mast cell
histamine release
