摘要
[目的]探讨饮水钙、镁摄入量对切除卵巢大鼠骨质量的影响。[方法]按2×2析因设计,以高镁低钙饮水 (钙5mg/L、镁60 mg/L,A1B1)、高镁正常钙饮水(钙65 mg/L、镁60 mg／L A2B1)、正常镁低钙饮水组(钙5 mg/L、镁30 mg/L, A1B2)、正常钙镁饮水组(钙65 mg/L、镁30 mg/L,A2B2)分别饲养去卵巢Wistar大鼠3个月,实验结束时测血清骨钙素及尿羟脯氨酸、肌酐含量;左侧股骨测骨长、骨重、骨钙镁含量;右侧胫骨作病理检查。[结果]饮水低钙可使骨矿物质含量降低,尿羟脯氨酸/肌酐升高,各组血清骨钙素浓度无差异;饮水高镁有相似作用,但不如低钙因素作用显著。骨病理检查结果由好到差依次为正常饮水组,高镁饮水组、低钙饮水组、高镁低钙饮水组。[结论]饮水高镁、低钙均可致去卵巢大鼠骨矿量减少、显微结构破坏,本研究未见饮水高镁、低钙摄入存在交互作用。
[ Objective ] To study the influence on bone mass of ovariectomized rats by magnesium and/or calcium water intake. [ Methods ] Three-month-old ovariectomized rats were fed with different types of drinking water： interaction group was fed with deficient-Ca and excessive-Mg water： 5 mg Ca/L, 60 mg Mg/L; Mg-excessive group with 60 mg Ca/L, 60 mg Mg/L; Ca-deficient group with 5 mg Ca/L, 30 mg Mg/L; normal group with 60 mg Ca/L, 30 mg Mg/L. After 3-month trial, the density of serum osteoealcin, hydroxyproline and creatine in urine was measured. Moreover, left femur was measured its length and weight, then toasted to ash and measured the content of Ca and Mg, and right tibia was taken for the pathologyical examination. [ Results ] The content of bone mineral was reduced, and urinary hydroxyproline/creatine was rised by Ca-deficient intake, though there was no statistical difference in the density of serum osteocalcin. The effect of excessive-Mg intake was weaker than that of Ca-deficient intake. It easily distinguished the difference in bone microstructure by pathological examination： the control group has the best microstructure, Mg-excessive group and Ca-deficient group has worse, and the interaction group has the worst one. [ Conclusion ] Both Ca-deficient and excessive-Mg are the factors that would reduce the content of bone mineral and destroy the microstructure of bone. The interaction between deficient-Ca and excessive-Mg in drinking water is not found.
出处
《环境与职业医学》
CAS
北大核心
2006年第2期141-143,共3页
Journal of Environmental and Occupational Medicine
关键词
低钙
高镁
骨质量
卵巢切除术
析因设计
deficient celcium
excessive magnesium
bone mass
ovariectomized rat
factorial design
