摘要
目的:观察三七总皂甙对大鼠脊髓损伤细胞凋亡的保护作用并分析其可能的作用途径。方法:实验于2005-06/11在华中科技大学同济医学院附属协和医院骨科实验室完成。实验共选取64只SD大鼠,随机分为损伤组和三七总皂甙组,每组32只,采用改良Allen’s重物打击法造成大鼠中度脊髓损伤,三七总皂甙组于伤后30min腹腔注射50g/L三七总皂甙(100mg/kg),伤后2,4h用50mg/kg,以后1次/d,剂量200mg/kg,连用12d。损伤组于伤后各时间点腹腔注射等体积生理盐水。观察比较治疗后不同时间大鼠行为评分、斜板试验、组织学检查及脊髓神经细胞凋亡指数变化。结果:64只SD大鼠全部纳入结果分析。①三七总皂甙组和损伤组大鼠在7d时行为评分分别为2.50±0.53、1.38±0.74,14d时分别为3.25±0.89、1.75±0.71,两组比较差异有显著性(t=-3.473,-3.742,P<0.05)。②三七总皂甙组与损伤组1,3,7,14d各时间点斜板最大角度差异均有显著性(t=-4.081,-4.235,-4.245,-4.687,P<0.05)。③苏木精-伊红染色光镜下发现脊髓组织病理学改变三七总皂甙组明显轻于损伤组。④损伤组大鼠脊髓损伤后1d在灰质及白质中均可见标记阳性细胞,损伤3d后凋亡指数渐增,7d达高峰;三七总皂甙组大鼠脊髓神经细胞凋亡指数较损伤组低,高峰提前,发生在损伤后3d,以后逐渐降低。两组比较差异均有显著性(t=4.196,2.627,7.732,3.547,P<0.05)。结论:三七总皂甙能抑制脊髓损伤神经细胞凋亡,对损伤脊髓的神经功能具有保护作用。
AIM： To investigate the protective effect and action mechanism of panax notoginseng saponin （PNS） on apoptosis in rats with spinal cord injury （SCI）.
METHODS： The experiment was completed at the Laboratory of Orthopedics, Union Hospital; Tongji Medical College, Huazhong University of Science and Technology from June to November 2005. Totally 64 SD rats were divided randomly into injured group and PNS group with 32 in each group. Moderate SCI of rats was induced by modified Allen＇s method. Rats in PNS group were injected intravenously with 100 mg/kg PNS （50 g/L） 30 minutes after injury, with 200 mg/kg PNS （50 mg/kg） 2 and 4 hours after injury once a day for 12 days. Rats in injured group were injected intravenously with the same volume of saline at each time point. Then, behavior scores, tilthoard test, histological examination and apoptotic index of nerve cell were measured at each time point before and after treatment.
RESULTS： All 64 SD rats were involved in the result analysis. ① Behavior scores of rats＇in PNS group and injured group were 2.50±0.53 and 1.38±0.74 on the 7^th day and 3.25±0.89 and 1.75±0.71 on the 14^th day, respectively, and there were significant differences between two groups （t=-3.473,-3.742,P 〈 0.05）. ② Maximal angle of tiltboard test was significantly different between PNS group and injured group on the 1^st, 3^rd, 7^thand 14^th days （t=-4.081, -4.235, -4.245, -4.687, P〈0.05）.③ Histological changes were slighter in PNS group than those in injured group with haematine-eosin staining under light microscope. ④ Labeling positive cells were observed in cinereum and white matter of rats in injured group 1 day after SCI, apoptosis index was increased 3 days later, and reached at peak 7 days later. Apoptosis index of rats was lower in PNS group than that tn injured group, the peak was existed 3 days later, and then it was decreased gradually. There was significant difference between two groups （t=4.196, 2.627, 7.732, 3.547, P 〈 0.05）.
CONCLUSION： PNS can inhibit apoptosis of nerve cell in SCI rats and has a protective effect on recovery of the nerve function.
出处
《中国临床康复》
CAS
CSCD
北大核心
2006年第15期62-64,共3页
Chinese Journal of Clinical Rehabilitation
