摘要
通过RT-PCR方法扩增β-酪蛋白基因,扩增产物插入质粒载体pET-28 a中构建成表达载体,转化入大肠杆菌BL-21,经IPTG诱导表达。利用限制性内切酶酶切图谱分析、DNA序列测定及SDS-PAGE,结果表明,成功地用RT-PCR方法从小鼠乳腺组织中克隆出β-CN基因并构建了重组β-CN的表达载体,并在大肠杆菌获得表达。
RT-PCR was used to amplify DNA of β-casein gene, which was inserted into pET-28a to construct the expression vector. The recombinant vector was transformed into BL21 and IPTG was used to induce expression. By restriction endonucleases mapping analysis and DNA sequencing, the constructed plasmid was the recombinant one. The expressed vector was successfully constructed and β-casein gene was exoressed in E. coll.
出处
《安徽农业大学学报》
CAS
CSCD
北大核心
2006年第2期180-182,共3页
Journal of Anhui Agricultural University
基金
安徽省自然科学基金项目(03043802)
安徽省高等学校青年教师科研项目(2004jq157)资助
