摘要
目的:通过观察脑泰方提取物对局灶性脑梗死大鼠血浆纤溶系统活性的影响,阐述脑泰方治疗局灶性脑梗死的作用机制。方法:将33只大鼠称重编号,按完全随机法分为正常对照组7只(灌胃生理盐水)、假手术组7只(灌胃生理盐水)、模型组7只(灌胃生理盐水)、脑复康组6只(灌胃质量分数为2.8%的脑复康溶液,每日剂量0.28g/kg)和脑泰方提取物组6只(灌胃脑泰方提取物,每日剂量2.8g/kg浸膏粉);通过神经功能缺失体征评分及脑组织病理形态学改变判定模型的成功,检测各组血浆组织型纤溶酶原激活物(t PA)及其抑制物(PAI)活性。结果:模型组大鼠血浆t PA活性明显低于正常对照组,PAI活性明显高于正常对照组;脑泰方提取物组血浆t PA活性和t PA/PAI比值明显升高,PAI活性基本恢复到正常对照组水平;脑泰方提取物和脑复康的作用基本一致。结论:脑泰方提取物能影响脑梗死大鼠血浆纤溶系统;其作用机制之一可能与保护血管内皮细胞功能、改变纤溶活性有关。
Objective： To observe the effect of Naotaifang extracts （脑泰方提取物） on plasma fibrinolytic activity of focal cerebral infarction in rats, and investigate the mechanisms of Naotaifang extracts in treatment of focal cerebral infarction. Methods： Thirty-three rats were weighted and put in order, then they were randomly divided into normal control group （n = 7）, sham-operated group （n = 7）, model group （n=7）, Naofukang （脑复康） group （n = 6） and Naotaifang extracts group （n=6）. The normal control group, sham-operated group and model group were given normal saline infused into stomach. Naofukang group was given 0.28g·kg^-1· d^-1 of 2.8% Naofukang solution infused into stomach. Naotaifang extracts group was given 2.8 g·kg^-1· d^-1 of Naotaifang extract powder. Neural function defect score and cerebral pathophysiology were used to assess the success of the model establishment. The levels of tissue plasmlnogen activator （t -PA） and plasminogen activator inhibitor （PAI） were detected in all the groups. Results.. The activity of t PA in model group was lower than that in the normal control group, while PAI was higher. In Naotaifang extracts group, the activity of t - PA and the ratio of t - PA/PAI increased significantly, PAI recovered to the normal level. The effect of Naofukang was similar to that of Naotaifang extracts. Conclusion ： Naotaifang can influence plasma fibrinolytic activity in rats with focal cerebral infarction by improving vascular endothelial function and fibrinolytic activity.
出处
《中国中西医结合急救杂志》
CAS
2006年第2期70-72,F0003,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
湖南省杰出中青年科技基金资助项目(00JZY2145)
关键词
脑梗死
局灶性
动物模型
脑泰方
脑复康
纤溶活性
focal cerebral infarction
animal model
Naotaifang
Naofukang
fibrinolytic activity
