摘要
目的 利用四氧嘧啶建立糖尿病小鼠模型，研究蛋白多糖F-苷肽（F-GC）对糖尿病小鼠血糖及肝、肾抗氧化功能的影响。方法 昆明小鼠禁食24h后，尾静脉注射42．5mg·kg^-1四氧嘧啶生理氯化钠溶液，5～7d后选择空腹血糖大于16．7mmol·L^-1的小鼠为造模成功者。随机分为5组，连续ig给药30d，F-苷肽小、中、大剂量组ig剂量分别为0．3、1、3g·kg^-1，阳性药物二甲双胍组ig剂量为0．2g·kg^-1，模型组与对照组ig等剂量生理氯化钠溶液。给药结束时测血糖，血清胰岛素；取肝、肾组织测抗氧化酶活性以及肝脏糖元含量。结果 糖尿病小鼠ig F-苷肽30d后，小、中、大剂量组血糖分别下降为8．14％、11．5％、14．68％，呈现一定的剂量效应关系；F-苷肽各组肝、肾超氧化物歧化酶（SOD）活性与模型组相比有显著提高（P〈0．05），与阳性药物二甲双胍组比较也显著提高（P〈0．05）。F-GC中、高剂量组的肾谷胱甘肽过氧化物酶（GSH-px）活性与模型组比较有显著提高（P〈0．01），其中F-GC中、大剂量组提高GSH-px活性的能力较二甲双胍强（P〈0．05）。肝GSH-px活性组问无显著变化。结论 F-苷肽对四氧嘧啶诱导的糖尿病小鼠有一定降血糖作用，其作用机制与其提高抗氧自由基酶类的活性和保护机体组织免遭过氧化损伤有关。
Aim To find out whether Fucoidan-Glycocalyx Compound （F-GC） can lower blood glucose,increase theplasma insulin concentration in Alloxan-induced diabetic mice, and improve the antioxidation in liver and kidney. Methods Alloxan induced diabetic mice received a dose of 42.5rag·kg^- 1 after a fast of 24 hours by intravenous injection on the tail. Diabetic mice were chosen 5-7days later, whose fasting blood glucose was above 16.7mmol· L^-1. Then diabetic mice were randomly divided into 5 groups： diabeticmedel group, three F-GC dose groups and positive drug group. Blood glucose, plasma insulin concentration and glycogen in liver were observed, The activity of SOD and GSH-px was also detected after being fed with F-GC and Metformin in the dose of 0.3,1,3g·kg^- 1 and 0.2g·kg^- 1 respectively for 30 days on end.Results The blood glucose of diabetic mice of F-GC groups dropped by 8.14%, 11.5%, 14.68% respectively,in a dosedependent manner. The activity of SOD of liver and kidney in each F-GC group increased more markedly than the diabetic model group （ P 〈 0.05）, so did Metformin group（ P 〈 0.05）. Activity of GSH-px in kidney also elevated significantly in the middle and high dose F-GC group, compared with diabetic model group（ P 〈 0.01 ） and Metformin group（P 〈 0.05） respectively. In liver, the activity of GSH-px changed a little. Conclusion F-GC exerts hypoglycemic effect on allowance-induced diabetic mice, which may be related to its effect of elevating activity of antioxidase and protecting tissues from damage of peroxidation.
出处
《解放军药学学报》
CAS
2006年第2期81-84,共4页
Pharmaceutical Journal of Chinese People's Liberation Army
基金
国家高技术研究发展计划(863)课题
No.2004AA2Z3341
