摘要
目的:探讨中草药槲皮素抑制人宫颈癌HeLa细胞增殖作用。方法:运用MTT法检测槲皮素对HeLa细胞增殖的抑制作用,流式细胞仪技术分析槲皮素对HeLa细胞增殖周期的影响。结果:10～160!mol/L浓度槲皮素均能抑制HeLa细胞的生长,且呈明显的时间、剂量依赖性关系。细胞周期分析显示槲皮素组G1期细胞比例明显增多,呈量效关系。结论:槲皮素抑制HeLa细胞增殖,可能通过使HeLa细胞停滞在G1期而诱导其调亡。
Objective： To investigate inhibition of quercetin （Que） on cell proliferation of the human uterine cervix cancer HeLa cells. Methods： Colorimetric MTF-assay was used to measure the Inhibition ratio of Que on human HeLa cells. The proliferation cycle of HeLa cells was determined using flow cytometry. Results： Que 10-160umol/L inhibited the proliferation of HeLa cells with different ratios and showed the time- and dose-dependent relationships. The augment of G1 ratio in the proliferation cycle of Hela cells presents a dose-dependent manner. Conclusion： Que can inhibit the proliferation of Hela cells and might induce the apoptosis by restrained cell cycle at G1.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2006年第8期436-438,共3页
Chinese Journal of Clinical Oncology
基金
湖北省计生委基金项目资助(编号:301140238)
