摘要
[目的]建立三香含漱液中延胡索乙素的含量测定方法。[方法]用乙醚提取三香含漱液中延胡索乙素,采用Zorbax ODS C18柱(250mm×4.6mm,5μm)为分析柱,以甲醇-水(67:33)为流动相,流速1.0ml/min,检测波长为280nm。[结果]延胡索乙素的浓度在5.78-46.25μg/ml范围内线性关系良好(r=0.99998),平均回收率为99.54%,RSD为0.23%。[结论]HPLC法操作简便、精密度好,可用于三香含漱液的质量控制。
[Objective]To establish the method for determining the content of tetrahydropalmatine in SanXiang Han Shu Ye. [Methods] Mobile phase: methanol-water (67:33) ; analytical column: Zorbax ODS C18 column (250mm × 4.6mm, 5μm) ; flow rate: 1.0ml/min; detective wavelength: 280nm. Then tetrahydropalmatine was extracted from SanXiang Han Shu Ye with alcohol. [Results] Good linear dependence was observed when the concentration of tetrahydropalmatine was within the range of 5.78-46.25μg/ml ( r = 0. 99998); the average recovery rate was 99.54 % with RSD being 0.23 %, [Conclusion]The HPLC method can be used for quality control of SanXiang Han Shu Ye with simple operation and accurate outcome.
出处
《广西中医学院学报》
2006年第1期41-43,共3页
Journal of Guangxi Traditional Chinese Medical University