摘要
目的:评价醋酸钙梯度法制备维A酸脂质体的可行性,并与薄膜法、逆相蒸发法进行质量比较。方法:采用薄膜法、逆相蒸发法和醋酸钙梯度法分别制备维A酸脂质体,用G-50葡聚糖凝胶柱分离,以紫外分光光度法和纳米激光粒度仪分别测定维A酸脂质体的包封率和粒径,以电子显微镜观察维A酸脂质体的形态。结果:以醋酸钙梯度法制备的维A酸脂质体包封率最高,达90.48%;其外观圆整均匀,平均粒径为35.8nm,4℃放置3mo质量稳定。结论:采用醋酸钙梯度法制备维A酸脂质体包封率较高,方法可行。
OBJECTIVE： To evaluate the feasibility of preparing tretinoin liposome by calcium acetate gradient method and to compare this method with the microfilm and the antiphase evaporation method. METHODS： Tretinoin liposome was prepared by microfilm method, antiphase evaporation method and calcium acetate gradient method, respectively, Sephadex G-50 was used for isolation and ultraviolet speetrophotometry was utilized to determine the entrapped efficiency and grain size of liposomal tretinoin. Eleetronmieroscope was applied to observe the shape of tretinoin liposome. RESULTS： The calcium acetate gradient technology achieved the highest entrapped efficiency of 90.48%, The liposomes had perfect shape with the mean diameter of 35.8nm and kept stable in quality at 4℃ for 3 months. CONCLUSION： The liposome with high entrapped efficieney could be prepared by calcium acetate gradient method, and the method is feasible.
出处
《中国药房》
CAS
CSCD
北大核心
2006年第8期579-581,共3页
China Pharmacy
关键词
醋酸钙梯度法
维A酸脂质体
包封率
质量评价
Calcium acetate gradient
Tretinoin liposome
Entrapped efficiency
Quality evaluation
