摘要
目的:制备抗人结肠癌相关抗原的单克隆抗体4D10,运用组织芯片技术研究其与人结肠癌组织反应的情况。方法:用人结肠癌细胞株LOVO免疫小鼠,用间接细胞ELISA和免疫组化的方法筛选抗结肠癌相关抗原的单抗。结果:获得一株能够稳定分泌抗人结肠癌相关抗原的杂交瘤细胞株。4D10能够与不同分期的结肠癌组织切片反应,且与正常组织和其他癌组织几乎不反应。结论:4D10对结肠癌的病理分期及预后结果都具有一定的临床参考价值。
Objective：To produce the mAb which specifically reacting with colorectal cancer confirmed by the tissue microarray technology. Methods ：The mice were immunize with LOVO, and the mAb were screened by indirect cell ELISA and immunohistochemistry. Results：A McAb was obtained which can react to the colon cancer, but very little or not to normal tissue and other caner tissues. Conclusion：A McAb which can react to the colon cancer have been obtained,and the McAb can be useful on clinic for diagnosis and prognosis of colorectal cancer.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2006年第4期353-355,359,共4页
Chinese Journal of Immunology
关键词
单克隆抗体
结肠癌
组织芯片
Monoclonal antibody
Colorectal cancer
Tissue microarray
