摘要
目的对8例HIV长期感染不进展(LTNP)及病程进展缓慢(SP)患者的病毒特异性细胞毒性T细胞(CTL)特征进行研究。方法设立队列研究,从中随机选出8例患者(4例LTNPs,4例SP)。通过采用重叠肽技术组建HIV-1B亚型全序列肽段,组建成三维肽库进行ELISPOT分析8例患者的HIV-1特异性细胞免疫反应。结果在大多数患者中存在较强的T细胞反应,特别对HIV-1病毒的pol、gag、nef蛋白的免疫反应比其他蛋白强。结论病毒特异性CTL免疫反应在HIV-1 LTNPs有较强并且很广泛的反应,这对于有效抑制病毒在人体内的生存有可能起到很大的作用,很可能是这些LTNPs及SP病程进展缓慢的主要原因之一。
Objective To study the magnitude and breadth of HIV specific T cell responses in 8 HIV infected long-term nonprogressors （LTNPs） and slow progressors （SP） who received contaminated blood. Methods PBMCs were tested in an Elispot assay against a matrix of peptides （15 mers overlapping by 11 amino acids） representing the full proteome of HIV clade B virus. The peptides were presented as 3 dimensional matrixes such that each peptide appears in 3 unique wells enabling the epitope to be identified. Results We found broad T cell responses in most donors, although in one individual, a strong response against only gag protein was observed and T cells responses to pol, gag and nef were more dominate than the others. Conclusion High frequency and broad HIV specific T cells detected in Chinese HIV infected LTNP and SP individuals are very likely associated with the control of the disease progression in those individuals.
出处
《首都医科大学学报》
CAS
2006年第2期143-146,共4页
Journal of Capital Medical University
基金
首都医科大学附属北京佑安医院与牛津大学分子医学研究所艾滋病合作项目:中国HIV/AIDS免疫特征研究(2005001)
国家十五攻关课题:国家艾滋病防治关键技术及产品研究(2004BT719A10)
关键词
人类免疫缺陷病毒
艾滋病
病毒特异性细胞毒性T细胞
HIV-1长期感染不进展者
human immunodeficiency virus （HIV）
acquired immunodeficiency syndrome（AIDS）
HIV-specific cytotoxic T lymphocytes
HIV-1 long-term nonprogressors （LTNPs）
