摘要
目的了解泌尿生殖道念珠菌病的菌群分布及体外抗真菌药敏情况。验证科玛嘉念珠菌显色培养基临床应用的可行性。方法40例菌株均来自北京友谊医院皮肤科和妇产科门诊,临床症状典型,真菌镜检阳性。应用科玛嘉念珠菌显色培养基进行菌种鉴定。利用微生物自动鉴定系统VITEK.YBC酵母菌鉴定卡,进行菌种鉴定。用酵母样真菌最低抑菌浓度(MIC)药敏试剂盒进行MIC值测定。结果对培养阳性的40例菌株,用科玛嘉念珠菌显色培养基菌种鉴定白念珠菌37例,非白念珠菌3例,用微生物自动鉴定系统YBC酵母菌鉴定卡菌种鉴定白念珠菌37例,近平滑念珠菌2例,隐球菌1例。对从临床分离的39株念珠菌进行7种抗真菌药物MIC敏感性测定,药敏检测结果二性霉素B为71.79%,酮康唑为100%,咪康唑为84.62%,伊曲康唑为41.03%,氟康唑为97.44%,5-氟胞嘧啶为97.44%,特比奈芬为84.62%。结论1)在生殖道念珠菌病的病原体中,白念珠菌仍为第一位致病菌,占念珠菌的94.87%(37/39)。2)科玛嘉念珠菌显色培养基和微生物自动鉴定系统YBC酵母菌鉴定卡在白念珠菌的鉴定方面吻合率可达到100%。
Objective To investigate the distribution and the drug-sensitivity of Candida SPP in the patients with uro-genital candidiasis in vitro in our hospital, and assess the clinical feasibility of to Candida Chromogenic ＆ Isolation Medium. Methods 40 clinical strains from patients with uro-genital candidiasis in the dermatology department and the gynecology department in our hospital. Candida Chromogenic ＆ Isolation Medium and microorganism automatic testing system were used to identify Candida species, and according to the NCCLS M-27A scheme with typical clinical symptoms, 40 strains were studied, including 39 isolates from patients, 1 quality control strain. Results 1） 37 of 40 isolates were candida albicans,2 of isolates were candida parapsilosis, and 1 of isolates was Cryptococcus laurentii. 2） Drug sensitivity rate of yeast to amphotericin B, ketoconazole, miconazole, itraconazole, fluconazole, cytosine, terbinafine were 71. 79%, 100%, 84. 62%, 41. 03%, 97. 44%, 97. 44%, 84. 62% respectively. Conclusion 1） Among the clinical strains, Candida albicans was the most important pathomycete, and it had a high frequency in uro-genital candidiasis. 2） indetification rate of Candida albicans was 100^ with Candida Chromogenic ＆ Isolation Medium coincides with microorganism automatic testing system.
出处
《首都医科大学学报》
CAS
2006年第2期266-269,共4页
Journal of Capital Medical University
关键词
生殖道念珠菌病
抗真菌药敏试验
菌群分布
genital eandidosis
antifungal drug susceptibility microbial sensitivity tests
distribution of Candida SPP
