摘要
目的本研究拟观察辛伐他汀对蛛网膜下腔出血后迟发性脑血管痉挛的影响,并探讨其机制。方法随机将新西兰白兔36只均分为假手术组、自发性蛛网膜下腔出血(SAH)组和 SAH+辛伐他汀组(n=12)。假注血组动物行枕大池假穿刺假注血,其他2组受试动物行枕大池穿刺2次注血的方法,制作迟发性脑血管痉挛模型。于0-6 d,经口给予SAH+辛伐他汀组家兔辛伐他汀5 mg／kg体重,其他动物给予等量的淀粉。所有受试动物在第1次穿刺后的第7天被处死, 比较不同组间基底动脉内径、内径与血管壁厚度之比(D／T)的变化;并应用免疫组织化学、逆转录- 聚合酶链反应(RT-PCR)的方法对家兔基底动脉的肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和 IL-6表达进行评价。结果与SAH组比较,SAH+辛伐他汀组动物血管痉挛明显缓解(P< 0．05)、其血管壁促炎细胞因子TNF-α、IL-1β和IL-6表达显著减少(P<0．05)。结论经口给予一定量的辛伐他汀(5 mg／kg体重)能明显缓解蛛网膜下腔出血后迟发性脑血管痉挛,其作用机制可能与辛伐他汀的抗炎作用有关。
Objective To observe the effect of simvastatin on delayed cerebral vasospasm （DCVS） following subarachnoid hemorrhage （SAH） and explore the mechanism. Methods Thirty-six New Zealand white rabbits were divided into three groups randomly （ n = 12 in each group） ：shamed-operation group, SAH group and SAH ＋ simvastatin group. No puncture was done in shamed group. DCVS models were made by the method of double-hemorrhage by injecting autologous arterial blood into the cisterna magna in SAH group and SAH ＋ simvastatin group. The animals in SAH ＋ simvastatin group were orally administrated with simvastatin （5 mg/kg） daily from day 0 to day 6. The equal quantity of starch was given in the same way to the animals in other 2 groups. The internal diameter and internal diameter/ wall thickness （D/T） of the basilar artery （BA） were measured. The expression of TNF-α, IL-1β and IL- 6 was detected by using immunohistochemical and RT-PCR methods after all animals were killed on the day 7. Results Cerebral vasospasm of BA in SAH ＋ simvastatin group was obviously alleviated with slight expression of TNF-α, IL-1β and IL-6 compared to that in SAH group （ P〈 0.05）. Conclusion Simvastatin （5 mg/kg） has an ability to reduce DCVS following SAH in rabbits, which may be correlated with its role of anti-inflammation.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2006年第5期551-553,共3页
Chinese Journal of Experimental Surgery
关键词
蛛网膜下腔出血
脑血管痉挛
辛伐他汀
炎症
Subarachnoid hemorrhage
Cerebral vasospasm
Simvastatin
Inflammation
