摘要
真核生物的翻译起始因子5A（eIF5A）是调控生物生长发育、衰老及环境适应等的重要因子。利用设计的小麦蛋白翻译起始因子5A基因的引物对小麦“中国春”基因组DNA和cDNA进行PCR扩增，并将扩增的特异片段回收、克隆和测序，从基因组DNA中得到长度分别为1679bp、1910bp两条带，从cDNA扩增得到1条636bp带，分别命名为eIF5a1（基因登录号：DQ167202）、eIF5a2（基因登录号：DQ167201）和eIF5a3。利用GeneRace方法得到eIF5a3（基因登录号：DQ167203）的全长为768bp。序列分析表明，eIF5a1、eIF5a2．具82．3％相似性，都形成636bp的转录产物，转录产物仅6个核苷酸差异。将eIF5a1、eIF5a2．和eIF5a3这3个序列的预测氨基酸序列进行比对，发现仅有1—2个氨基酸的差异，证实它们为eIF5A基因家族的成员。进化分析表明它们与报道的玉米、水稻、西红柿、烟草的eIF5A基因序列的遗传关系最近。进一步研究表明eIF5a2位于2B染色体上，并用半定量RT-PCR研究了小麦eIF5A基因的表达情况。
Eukaryotic translation initiation factor 5A （eIF5A） is involved in cell proliferation, senescence, development and environmental response. In this paper, DNA and cDNA of Chinese Spring was amplified by PCR with special primers, two special fragments of 1 679 bp and 1 910 bp were obtained with DNA as template and one special fragment of 636 bp was yielded with cDNA as template. Which were named eIF5a1 （GenBank number： DQ167202）, eIF5a2 （GenBank number： DQ167201 ） and eIF5a3, respectively. The complete sequence （768 bp） of eIF5a3 （GenBank number： DQ167203） was obtained using GeneRacer kit. Nucleotide sequence analysis shows that elFSal and eIF5a2 share identical transcriptions of 636 bp which have only 6 nucleotides difference. Putative amino acid sequences analysis revealed that there was only one or two amino acid difference in eIF5a1, eIF5a2 and eIF5a3. Phylogenic analysis indicated that the eIF5A of wheat were relation to corn, rice, tomato and potato closely. In additional, eIF5a2 is located on chromosome 2B using Langdon D-genomic disomic substitution lines and the expression of eIF5A was also investigated by Semi-quantitative PCR.
出处
《遗传》
CAS
CSCD
北大核心
2006年第5期571-577,共7页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:30170502)~~
