摘要
目的:评价雷帕霉素滴眼液与环孢霉素A滴眼液联合应用对大鼠角膜移植免疫排斥反应的抑制作用。方法:建立大鼠穿透性角膜移植动物模型,以SD大鼠为受体,Wistar大鼠为供体,分为A,B,C,D4组。手术后2d术眼分别滴用滴眼液基质100μL,2g/L雷帕霉素滴眼液100μL,10g/L环孢霉素A滴眼液100μL,2g/L雷帕霉素滴眼液50μL+10g/L环孢霉素A滴眼液50μL,4次/d,连续用药至排斥反应发生。对角膜植片进行临床观察,记录植片存活时间,并进行组织学检查。结果:各治疗组显著延长角膜植片存活时间(P<0.01),联合用药的D组效果更佳,优于各单药物治疗组B组和C组(P<0.05,P<0.01)。组织学发现,术后14d各治疗组炎细胞浸润、新生血管形成及水肿程度明显轻于对照组。结论:局部联合应用雷帕霉素滴眼液与环孢霉素A滴眼液能显著延长角膜植片存活时间,抑制大鼠角膜移植排斥反应,具有协同作用。
AIM: To evaluate the combined effect of rapamycin ophthalmic solution and cyclosporin A ophthalmic solution on allogeneic transplantation in a rat model.
METHODS: SD rats were used as recipients, and Wistar rats were used as donors. The animals were randomly assigned to 4 groups. Group A: control (ophthalmic solution solvent 100μL); group B: 2g/L rapamycin ophthalmic solution 100μL; group C: 10g/L cydosporin A ophthalmic solution 100μL; group D: 2g/L rapamycin ophthalmic solution 50μL and 10g/L cyclosporin A ophthalmic solution 50μL, 4 times every day. The treatment was started on 2d after operation, and the animals were administered until rejection. The grafts were inspected by slit-lamp microscope and the corneal survival time was recorded. The pathologic changes were measured by light microscope.
RESULTS: Graft survival in each treated group was significantly prolonged compared with the control group (P〈 0.01). However, the combined therapy (group D) was significantly superior compared with group B and C (P〈0.05, P〈 0.01). The histopathological findings showed that the inflammation cells, neovascularity in each treated group were significantly fewer than that in control group at 14d after operation.
CONCLUSION: This is the first study on the efficacy of a double drug regimen with rapamycin ophthalmic solution and cyclosporin A ophthalmic solution for the control of acute corneal allograft rejection. It indicats that the combined therapy produced synergistic effect.
出处
《国际眼科杂志》
CAS
2006年第2期271-274,共4页
International Eye Science
关键词
大鼠
角膜移植
免疫排斥
雷帕霉素
环孢霉素A
滴眼液
rats
keratoplasty
penetrating
immunologic rejection
rapamycin
cyclosporin A
ophthalmicsolution