摘要
目的:探讨磷脂酰肌醇-3激酶(phosphatidylinositol3-kinase,PI3K)在缺氧诱导的人视网膜色素上皮(retinalpigmentepithelium,RPE)细胞增生中的作用。方法:利用CoCl2法建立培养细胞缺氧模型。在缺氧条件下培养人RPE细胞1,4,8,12和24h,流式细胞仪检测RPE细胞的增生活性,RT-PCR和Westernblot检测磷酸化PI3KmRNA和蛋白的表达水平。用30μmol/LPI3K特异性阻断剂LY294002处理RPE细胞,作为阻断实验组。结果:随缺氧时间延长,RPE细胞增生活性逐渐增高,磷酸化PI3KmRNA和蛋白表达水平逐渐增高。LY294002处理组较对照组RPE细胞增生活性显著降低(P<0.05)。结论:缺氧诱导的RPE细胞增生部分是通过PI3K信号转导通路实现的。
AIM： To investigate the role of phosphatidylinositol 3-kinase （PI3K） signal transduction pathway in the proliferation of cultured human retinal pigment epithelial （RPE） cells under hypoxic condition.
METHODS： A cell model of hypoxia was established using Cobalt Chloride. RPE cells treated with/without the specific inhibitor of PI3K, LY294002 at the concentration of 30μmol/L, were cultured under hypoxia for 1,4,8, 12 and 24h. RPE cells proliferation activity were detected by flow cytometry （FCH）. The activity of phosphophorylation PI3K were observed with reverse transcription ploymerase chain reaction （RT-PCR） and Western-blot method.
RESULTS： The proliferation activity and the level of phosphophorylation PI3K of RPE cells were time-dependently increased with hypoxia. The proliferation activity of RPE cells in the group treated with LY294002 was significantly decreased compared with the control group（P〈0.05）.
CONCLUSION： PI3K activation is an essential component of the signal transduction pathway to increase proliferation in RPE cells under hypoxia.
出处
《国际眼科杂志》
CAS
2006年第2期306-310,共5页
International Eye Science
基金
中国国家自然科学基金资助项目(No.30371516)
教育部留学回国人员科研启动基金资助项目(2004)
第四军医大学科技创新工程资助项目(No.CX02A021)~~
