摘要
目的用不同方法去除猪主动脉瓣的细胞并比较效果,为组织工程瓣膜提供良好的实验材料。方法采用十二烷基硫酸钠(SDS)、脱氧胆酯酸钠(SD)、曲拉通(Triton)X-100制备猪主动脉瓣脱细胞基质。标本进行大体、光镜、电镜观察,DNA提取、力学性能的检测并比较。结果SD无法完全脱除主动脉瓣瓣根内的细胞。TritonX-100和SDS可以完全脱除瓣膜细胞。TritonX-100较好的保持了主动脉瓣胶原纤维和弹性纤维的原有排列和分布,并有较好的力学性能。而SDS对瓣叶和瓣根血管壁中的纤维支架都造成了一定的损伤。SDS和TritonX-100脱细胞时间无明显差别。结论TritonX-100可以成功脱除猪主动脉瓣细胞,并较好的保持了主动脉瓣的结构和力学性能。
Objective To remove cellular compenents from porcine aortic valve with different reagents, providing acellular tissue matrix（ACTM） scaffolds for tissue engineering of heart valve. Methods Different detergents （TritonX-100,Sodium dedecyl sulfate and sodium deoxycholate） and enzyme（trypsin） were used to remove cells and its, compenents from porcine aortic valves, respectively. According to the different detergents, specimens of porcine aortic valve were divided into three groups（ Sodium dedecyl sulfate, sodium deoxyeholate, and Triton X-100）. Specimens were obsexved grossly uncroscopically. Haematoxylin-eosin and DNA assay was performed to confirm the removal of cells. Transmission electron microscope was used to observe the integrity of collagen and elastin. The changes of mechanical propeeties were also studied. Results The cells were remeved effectively from cusps and roots of porcine aortic valves by Triton X-100 and sodium dedecyl sulfate in 96 h. Sodium deoxycholate could not effectively remove the cells from the root of aortic valve. Treatment with SDS disrupted collagen fiher structure of porcine aortic valve, resulting in poor mechanical properties. Treatment with TritonX-100 result in an acellular porcine aortic valve matrix with retention of near normal structure and mechanical properties. Conclusion Porcine aortic valve can be successfully decellularised with retention of near normal structure and mechanical properties by Triton X-100.
出处
《中华胸心血管外科杂志》
CSCD
北大核心
2006年第2期127-129,共3页
Chinese Journal of Thoracic and Cardiovascular Surgery
基金
国家自然科学基金(30271292)
陕西省卫生厅科学基金(2045)资助
关键词
心脏瓣膜
组织工程
胰蛋白酶
聚乙烯二醇类
脱细胞基质
Heart valves Tissue engineering Trypsin Polyethylene glycols Acellular tissue matrix
