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高效液相色谱法分离和测定知母不同炮制品中萨尔萨皂甙元

SEPARATION AND DETERMINATION OF SARSASAPOGENIN IN DIFFERENT PROCESSED PRODUCT OF AHEMARRHENA ASPHODELOIDES BUNGE BY HPLC
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摘要 首次用反相高效液相色谱法(RP-HPLC)分离和测定了知母不同炮制品中萨尔萨皂甙元,建立了中药中萨尔萨皂甙元分离、测定的色谱方法.色谱条件:ODS柱,以甲醇为流动相,以RID-6A型示差折光检测器检测.本方法为知母不同炮制品的质量研究提供了科学依据. Sarsasapogenin in different processed product of Ahemarrhena asphodeloidesBunge were separated and determined by RP--HPLC for the first time.A chromatographicmethod for the separation and the determination of the sarsasapogenin in Chinese medicinalherb was established.The chromatographic conditions are shown as the following:ODS column with methanol as mobile phase,and eluates were monitored with a RID-6Adetector,This provides a scientific basis of quality study of different processed product of the Anemarrhena asphodeloides Bunge.
出处 《宁夏大学学报(自然科学版)》 CAS 1996年第2期29-31,35,共4页 Journal of Ningxia University(Natural Science Edition)
关键词 知母 萨尔萨皂甙元 炮制品 高效液相色谱 分离 Anemarrhena asphodeloides Bunge sarsasapogenin processed product HPLC
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