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实时荧光定量聚合酶链反应技术及应用 被引量：2

Real-time quantitative PCR technology
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作者 赵紫琴 陆凤先
机构地区 天津医科大学病理生理教研室
出处 《中国药物与临床》 CAS 2006年第4期248-251,共4页 Chinese Remedies & Clinics
基金 国家自然科学基金资助项目(30370682) 天津市科技发展计划基金资助项目(05YFGDSF02700)
关键词 实时荧光定量聚合酶链反应技术 耐热DNA聚合酶 Green PCR) RQ-PCR 荧光标记引物 DNA嵌合剂 杂交探针 SYBR CHAIN
分类号 R450 [医药卫生—治疗学]
  • 相关文献

参考文献15

  • 1Kutyavin Ⅳ,Afonina IA,Mills A,et al.3'-Minor groove binderDNA probes increase sequence specificity at PCR extension temperatures.Nucleic Acids Res,2000,28 (2):655-661.
  • 2Bustin SA.Quantification of mRNA using real-time reverse transcription PCR (RT-PCR):trends and problems.J Mol Endocrinol,2002,29(1):23-39.
  • 3Wada T,Maeda S,Tamaru A,et al.Dual-probe assay for rapid detection of drug-resistant mycobacterium tuberculosis by realtime PCR.J Clin Microbiol,2004,42(11):5277-5285.
  • 4Rapossi V,Burm BEA,Cofoi S,et al.Antiproliferative effect in chronic myeloid leukaemia cells by antiscnse peptide nucleic acids.Nucleic Acids Res,2002,30 (17):3712-3721.
  • 5Petersen K,Vogel U,Rockenbauer E,et al.Short PNA molecular beacons for real-time PCR allelic discrimination of single nucleotide polymorphisms.Mol Cell Probes,2004,18(2):117-122.
  • 6Xi C,Balberg M,Boppart SA,et al.Use of DNA and peptide nucleic acid molecular beacons for detection and quantification of rRNA in solution and in whole cells.Appl Environ Microbiol,2003,69(9):5673-5678.
  • 7Mackay IM,Arden KE,Nitsche A,et al.Real-time PCR in virology.Nucleic Acids Res,2002,30(6):1292-1305.
  • 8Thelwell N,Millington S,Solinas A,et al.Mode of action and application of scorpion primers to mutation detection.Nucleic Acids Research,2000,28 (19):3752-3761.
  • 9Van der Velden VH,Hochhaus A,Cszzaniga G,et al.Detection of minimal residual disease in hematologic malignancies by realtime quantitative PC R:principles,approaches,and laboratory aspects.Leukemia,2003,17(6):1013-1034.
  • 10Aryee EA,Bailey RL,Natividad-Sancho A,et al.Detection,quantification and genotyping of herpes simplex virus in cervicovaginal secretions by real-time PCR:a cross sectional survey.Virol J,2005,2(1):61.

同被引文献33

  • 1丰培金,孙艳侠.鱼类免疫球蛋白生物合成的遗传控制研究综述[J].安徽农业科学,2005,33(8):1484-1485. 被引量：3
  • 2罗建蓉,贾永前.实时荧光定量PCR在监测微量残留性白血病中的应用[J].华西医学,2006,21(2):417-418. 被引量：4
  • 3周成江,周立社,吴刚,邵国,苏燕.实时荧光定量PCR的研究进展及其应用[J].包头医学院学报,2007,23(2):204-207. 被引量：8
  • 4周净,王云龙,李智涛,李玲玲.JEV-DNA实时荧光定量标准品的构建[J].生物技术通报,2007,23(4):178-180. 被引量：5
  • 5[3]Atya M,Shergill I S,Williamson M,et al.Basic principles of real-time quantitative PCR[J].Expert RevMol Diagn,2005,5 (2):209-213.
  • 6[4]Wall S J,Eoward D R.Quantitative reverse transcription-polymerase chain reaction (RT-PCR):A comparison of primer-dropping,competetive and real-time RT-PCR[J].Anal Biochem,2002,300:269-273.
  • 7[6]Ernard S,Wittwer C T.Real time PCR technology for cancer diagnostics[J].Clin Chem,2002,48(8):1178-1185.
  • 8[9]Mas V R,Maluf D G,Archer K J,et al.Study of mRNA growth factors in urinary cells of kidney transplant recipients as predictors of chronic allograft nephropathy[J].Transplantation,2005,80 (12):1686-1690.
  • 9[10]Suziu N,Yoshid A,Naknor Y.Quantitative analysis of multi-species oral biofilms by TaqMan real-time PCR[J].Clin MedRes,2005,3(3):176-185.
  • 10[11]Schmitgen T D,Zakrajsek A,Millsa J,et al.Quantitative reverse transcription polymerase chain reaction to study mRNA decay:comparison of endpoint and real time methods[J].Anal Biochem,2000,285(2):194-204.

引证文献2

二级引证文献10

中国药物与临床
2006年 第4期

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