摘要
目的在体外通过细胞培养,初步研究5-氟尿嘧啶和奥沙利铂对人结肠癌细胞FasLmRNA表达及其诱导淋巴细胞凋亡的影响,为合理应用化疗药物,全面评价其对机体的影响提供新的思路。方法应用逆转录-聚合酶链反应(RT-PCR)法检测5-氟尿嘧啶和奥沙利铂作用前后人结肠癌SW480细胞FasLmRNA的变化;应用流式细胞术检测5-氟尿嘧啶和奥沙利铂作用前后SW480细胞诱导淋巴细胞凋亡率的变化。结果RT-PCR法检测结果显示,5-氟尿嘧啶和奥沙利铂处理后较处理前SW480细胞FasLmRNA表达水平均明显高于对照组(P<0.01);而且FasLmRNA表达水平随5-氟尿嘧啶、奥沙利铂作用浓度增加显著上调,5-氟尿嘧啶、奥沙利铂不同浓度组比较均有显著性差异(P<0.01);流式细胞术检测结果表明,随5-氟尿嘧啶和奥沙利铂作用浓度升高,SW480细胞诱导淋巴细胞凋亡率明显升高,不同浓度组比较均有显著性差异(P<0.01)。结论5-氟尿嘧啶、奥沙利铂在一定时间内均可上调人结肠癌SW480细胞FasLmRNA的表达,而且这种上调作用具有功能性,能协助肿瘤细胞反向攻击淋巴细胞。
Objective： To study the effect of 5 -fluorouracil and oxaliplatin on Fas Ligand mRNA expression of human colon cancer cells line SW480 and apoptosis of lymphocyte induced by it with cell culture. Methods： Different concentrations of 5 - fluorouracil and oxaliplatin have been used in the cultured human colon cancer cells line SW480. The change of Fas Ligand mRNA was detected by the reverse transcription polymerase chain reaction method （ RT - PCR） and apoptosis rate of lymphocyte induced by SW480 cell was measured by flow cytometry analysis. Results： Results of RT - PCR revealed that the higher concentration 5 - fluorouracil or oxaliplatin raised Fas Ligand mRNA expression on SW480 cell; Results of flow cytometry showed that the higher concentration 5 - fluorouracil or oxaliplatin increased apoptosis rate of lymphocyte induced by SW480 cell, the comparison of different concentrations groups of 5 - fluorouracil or oxaliplatin had statistical significance. Conclusion：5 - fluorouracil and oxaliplatin all can upregulate functional Fas Ligand mRNA expression of human colon cancer cells line SW480 and the upregulative effect can assist cancer cells to counterattack lymphocyte.
出处
《现代肿瘤医学》
CAS
2006年第5期533-535,共3页
Journal of Modern Oncology
