摘要
Aiming at development of transformation methods for alkalophilic Bacillus pseudofirmus JCM 9141 (growth at pH>10), screening conditions of transformants by using antibiotics were first examined to overcome the antibiotic inactivation at high pH. Then, different transformation methods including competent cells, protoplast and electroporation were studied, respectively. The transformation efficiencies of competent cells and protoplast were low, while a modified electroporation method achieved higher transformation efficiency under 3000 V·cm -1. This method was also applicable to other alkalophilic Bacillus sps.
Aiming at development of transformation methods for alkalophilic Bacillus pseudofirmus JCM 9141 （growth at ph〉10）, screening conditions of transformants by using antibiotics were first examined to overcome the antibiotic inactivation at high pH. Then, different transformation methods including competent cells, protoplast and electroporation were studied, respectively. The transformation efficiencies of competent cells and protoplast were low, while a modified electroporation method achieved higher transformation efficiency under 3000 V · cm^-1. This method was also applicable to other alkalophilic Bacillus sps.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2006年第4期922-926,共5页
CIESC Journal
基金
国家自然科学基金项目(20176025)
国家教育部博士点基金项目(20020003057).~~
