摘要
一步成苗分化的魔芋组培苗长至3～4cm，分株切割，置入诱导试管芋的不同配比的6种培养基中培养，在不同的光、温条件下培养约20d，在培养基MS＋NAA 1mg／L＋PP3331mg／L、光照1500～2000lx、温度25～30℃的培养条件下，试管内大量形成试管芋。试管芋播种于大田，成活率达100％，5～6个月收获150～200g的球茎。
Under different temperature and light conditions, 3 -4 cm high plantlets in vitro of Amorphophallus rivieri Durieu were cultured in 6 combinations of culture media for 20 days.Results indicated that MS＋ 1.0 mg/L NAA＋ 1.0 mg/L PP333 was the best for mass-producing corms in vitro at 1500 - 2000 lx and 25 - 30 ℃ .The survival rate of corms in vitro transplanting in the fields reached nearly 100 % and Tube-tubers in the field can produce large solid corms with 150 - 200 g/l corm after 5 - 6 months.
出处
《西南农业学报》
CSCD
2006年第2期280-282,共3页
Southwest China Journal of Agricultural Sciences
基金
云南省科技厅"十五"攻关项目(2001NG59)
关键词
魔芋
试管芋
NAA
PP333
光照
温度
Arnorphophallus rivieri Durieu
corms in vitro
NAA
PP333
light
temperature
