摘要
使用以菊粉为惟一碳源的培养基从自然界中分离出6株产菊粉酶较强的菌种,其中黑曲霉SL-08还可以最大程度地提高塞尔维亚酵母Z-06的发酵活力和耐酒精能力,通过紫外和亚硝基胍诱变,得到菌株SL-09,酶活提高近两倍。以菊芋粉为底物,利用黑曲霉SL-09和塞尔维亚酵母Z-06采用同步糖化与发酵法,30℃发酵60 h,使发酵醪酒精体积分数达到19.0%,转化率为理论转化率的86%。
Six inulinase producing fungi strains were isolated from soil by a medium using inulin as unique carbon source. Among them, Aspergillus niger SL-08, which strongly enhanced the fermentation activity and ethanol tolerance of Saccharomyces cerevisiae Z-06, was subjected to UV and NTG mutagenesis. As a result, Aspergillus niger SL-09 was obtained and the enzyme activities were enhanced to a level, which was about two folds higher than that of the original strain. 19. 0% of ethanol was produced from Jerusalem artichoke flour within 60 h of fermentation by simultaneous saccharification and fermentation using Aspergillus niger SL-09 and Saccharomyces cerevisiae Z-06. The conversion yield from Jerusalem artichoke to ethanol reached 86% of the theoretical value.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2006年第2期83-87,92,共6页
Journal of Food Science and Biotechnology
关键词
菊芋
酒精
黑曲霉
菊粉酶
Jerusalem artichoke
ethanol
Aspergillus niger
inulinase
