摘要
以菌丝生物量及胞外多糖(exopolysaccharides,EPS)含量为指标对大球盖菇产胞外多糖液体培养基组成和发酵条件进行了优化。结果表明,最适碳源是麦芽糖,最适氮源是酵母膏,正交试验确定最佳培养基组成为马铃薯150 g/L,麦芽糖20 g/L,酵母膏1 g/L,KH2PO41 g/L,MgSO4.7H2O 2.5 g/L。最佳发酵条件为28℃,摇床转速160 r/min,起始pH值6.5,装液量100 mL/250 mL、接种量10%,发酵时间6 d。在此条件下,大球盖菇菌丝生物量及EPS含量分别比对照增加了31.8%和51.6%。
Optimization of liquid culture medium components and fermentation condition to produce extracellular polysaccharides （EPS） was carried out with hypha biomass and EPS content as target. The results showed that optimum carbon and nitrogen sources were maltose and yeast extract respectively. Orthogonal experiment has confirmed that the optimum medium was 150 g/L of potato, maltose 20 g/L, yeast extract 1 g/L, KH2PO4 lg/L, MgSO4. H2O 2.5 g/L. And the optimum temperature was 28 ℃ , rotate at 160 rpm, with initial pH at 6.5, filling amount at 100 mL/250 mL, 10% inoculation, and ferment for 6 days. Under these conditions the output of hypha biomass and EPS increased by 31.8 % and 51.6 % respectively as compared with the control.
出处
《微生物学杂志》
CAS
CSCD
2006年第2期45-49,共5页
Journal of Microbiology
基金
山东省教育厅资助项目(J01K32)
关键词
大球盖菇
生物量
胞外多糖
培养条件
Stropharia rugoso-annulata
extracellular polyaccharides
biomass
culture condition
