摘要
目的建立一种研究离子通道的有效模型。方法采用Lipofacta mine2000脂质体将人的超极化激活的环核苷酸门控(HCN)基因转染人胚胎肾(HEK)293细胞,利用全细胞膜片钳技术检测克隆人HCN2基因的表达。结果pcDNA3-hHCN2真核表达载体转染HEK293细胞3d后,全细胞膜片钳技术记录到克隆人HCN2基因编码的通道电流。结论全细胞膜片钳技术稳定、可靠,可为开展克隆离子通道结构和功能关系研究提供基础。
Objective To create a model for studying ionic channels. Methods pcDNA3- hHCN2 was transfected with lipofectamine 2000 into HEK293 Cells. The expression of hHCN2 gene was detected by whole-cell patch clamp. Results Whole-cell patch clamp recorded ionic currents of HEK293 cells after transfected pcDNA3-hHCN2 three days later. Conclusion The technique is reliable and consistent, which will be helpful for studying the relationship between the structure and function of cloned ionic channels.
出处
《江苏医药》
CAS
CSCD
北大核心
2006年第5期451-452,共2页
Jiangsu Medical Journal
关键词
人胚胎肾293细胞
脂质体
基因
Human embryonic kidney（HEK） 293 cell
Liposome
Gene
