摘要
利用半滑舌鳎胚胎为试验材料,研究了Cu、Hg、Zn、Cd、Pb5种重金属对胚胎发育的毒性效应。胚胎在0.01、0.08和0.1mg/LCu2+溶液中孵化率较低,与对照组差异显著(P≤0.05);除0.125、0.25和0.5mg/LCd2+会促进胚胎发育速率外,其余4种重金属都会在不同程度上减慢胚胎孵化速率。重金属离子在胚胎发育过程中引起了各种各样的畸形现象,如眼睛残缺、胚胎异常死亡、胚体解体模糊、胚胎尾部弯曲;初孵仔鱼不能破膜而出,初孵仔鱼脊椎弯曲成“S”形、“V”形、“L”形等。综合孵化率、仔鱼畸形率和胚胎畸形程度等指标可以得出5种重金属对胚胎的毒性大小依次为:Cu>Hg>Cd>Zn>Pb。还研究了Hg、Cu、Cd、Pb、Zn等5种重金属对半滑舌鳎初孵仔鱼、10d仔鱼和20d稚鱼急性(96h)毒性影响,并计算了几种重金属对半滑舌鳎仔稚鱼的半数致死浓度(LC50)和安全浓度。
To help define the tolerance of marine environmental stress exerted by heavy metals, embryos, larvae of Cynoglossus semilaevis Günther were incubated at different levels of copper, mercury, lead, zinc and cadmium solutions, respectively. Embryos incubated at 0. 01 mg/L,0.08 mg/L,0, l mg/L copper solutions showed low hatching rate, which significantly different from the control （P~〈0. 05）, other heavy metals didn＇t affect the hatching rate of embryos notably, but the newly-hatched larvae in all experimental solutions exhibited significant difference from the control for the high deforming rate. Embryos rinsed in 0. 125 mg/L,0. 25 mg/L,0.5 mg/L cadmium solutions could boost the development speed. Malformation of embryos were observed during the experiment, such as monothphmia, cyclopia, embryo death, larvae have V-shaped, S-shaped, L-shaped vertebral abnormality, the larvae can＇t hatched from the embryos, and so on. The 50% lethal concentration and safety concentration of these metals of 24 h,48 h,72 h and 96h for newly hatched larvae, larvae of 10days after hatching, larvae of 20days after hatching are calculated to help establish water criterion and protect marine environment. Larvae were sensitive to metals at high concentrations, many died soon after entering the solution. The abnormal larvae affected by the metals showed the curled vertebra and inconsistent swimming behavior.
出处
《海洋水产研究》
CSCD
北大核心
2006年第2期33-42,共10页
Marine Fisheries Research
基金
国家863高技术研究发展计划项目(2002AA603011和2004AA603320)资助
关键词
半滑舌鳎
重金属
胚胎和仔稚鱼
毒性效应
Cynoglossus semilaevis Günther Heavy metals Embryos and Larvae Toxic effects
