期刊文献+

Oxidative damage,pro-inflammatory cytokines,TGF-αand c-myc in chronic HCV-related hepatitis and cirrhosis 被引量:5

Oxidative damage,pro-inflammatory cytokines,TGF-αand c-myc in chronic HCV-related hepatitis and cirrhosis
下载PDF
导出
摘要 AIM: To assess whether a correlation exists between oxidative DNA damage occurring in chronic HCV-related hepatitis and expression levels of pro-inflammatory cytokines, TGF-α and c-myc. METHODS: The series included 37 patients with chronic active HCV-related hepatitis and 11 with HCV-related compensated cirrhosis. Eight-hydroxydeoxyguanosine in liver biopsies was quantified using an electrochemical detector. The mRNA expression of TNF-α, IL-1β, TGF-α and c-myc in liver specimens was detected by semiquantitative comparaUve RT-PCR. RESULTS: TNF-α levels were significantly higher in hepatitis patients than in cirrhosis patients (P=0.05). IL-1β was higher in cirrhosis patients (P=0.05). A sig- nificant correlation was found between TNF-α and staging (P= 0.05) and between IL-1β levels and grading (P=0.04). c-myc showed a significantly higher expression in cirrhosis patients (P=0.001). Eight-hydroxydeoxyguanosine levels were significantly higher in cirrhosis patients (P=0.05) and in HCV genotype 1 (P=0.03). Considering all patients, 8-hydroxydeoxyguanosine levels were found to be correlated with genotype (P=0.04) and grading (P=0.007). Also multiple logistic regression analysis demonstrated a significant correlation among the number of DNA adducts, TNF-α expression and HCV genotype (P= 0.02). CONCLUSION: In chronic HCV-related liver damage, oxidative DNA damage correlates with HCV genotype, grading and TNF-α levels. As HCV-related liver damage progresses, TNF-α levels drop while IL-1β and c-myc levels increase, which may be relevant to liver carcinogenesis. AIM: To assess whether a correlation exists between oxidative DNA damage occurring in chronic HCV-related hepatitis and expression levels of pro-inflammatory cytokines, TGF-αand c-myc. METHODS: The series included 37 patients with chronic active HCV-related hepatitis and 11 with HCV-related compensated cirrhosis. Eight-hydroxydeoxyguanosine in liver biopsies was quantified using an electrochemical detector. The mRNA expression of TIMF-α, IL-1β, TGF-αand c-myc in liver specimens was detected by semi-quantitative comparative RT-PCR. RESULTS: TNF-αlevels were significantly higher in hepatitis patients than in cirrhosis patients (P=0.05). IL-1βwas higher in cirrhosis patients (P=0.05). A significant correlation was found between TNF-αand staging (P=0.05) and between IL-1βlevels and grading (P=0.04). c-myc showed a significantly higher expression in cirrhosis patients (P=0.001). Eight-hydroxydeoxyguanosine levels were significantly higher in cirrhosis patients (P=0.05) and in HCV genotype 1 (P=0.03). Considering all patients, 8-hydroxydeoxyguanosine levels were found to be correlated with genotype (P=0.04) and grading (P=0.007). Also multiple logistic regression analysis demonstrated a significant correlation among the number of DNA adducts, TNF-αexpression and HCV genotype (P=0.02). CONCLUSION: In chronic HCV-related liver damage, oxidative DNA damage correlates with HCV genotype, grading and TNF-αlevels. As HCV-related liver damage progresses, TNF-αlevels drop while IL-1βand c-myc levels increase, which may be relevant to liver carcinogenesis.
出处 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第13期2065-2069,共5页 世界胃肠病学杂志(英文版)
基金 Supported by PRIN grants from the Italian Ministry of Science and Technology, No. 2003063143-006
关键词 Oxidative DNA damage Chronic HCV-related hepatitis Inflammatory mediators 氧化损伤 细胞活素 肝硬化 丙型肝炎疾病
  • 相关文献

参考文献38

  • 1[1]Basaga HS.Biochemical aspects of free radicals.Biochem Cell Biol 1990; 68:989-998
  • 2[2]Adelman R,Saul RL,Ames BN.Oxidative damage to DNA:relation to species metabolic rate and life span.Proc Natl Acad Sci USA 1988; 85:2706-2708
  • 3[3]Kuchino Y,Mori F,Kasai H,Inoue H,Iwai S,Miura K,Ohtsuka E,Nishimura S.Misreading of DNA templates containing 8-hydroxydeoxyguanosine at the modified base and at adjacent residues.Nature 1987; 327:77-79
  • 4[4]Olinski R,Gackowski D,Rozalski R,Foksinski M,Bialkowski K.Oxidative DNA damage in cancer patients:a cause or a consequence of the disease development? Mutat Res 2003; 531:177-190
  • 5[5]de Groot H.Reactive oxygen species in tissue injury.Hepatogastroenterology 1994; 41:328-332
  • 6[6]Floyd RA.The role of 8-hydroxyguanine in carcinogenesis.Carcinogenesis 1990; 11:1447-1450
  • 7[7]Farinati F,Cardin R,Degan P,De Maria N,Floyd RA,Van Thiel DH,Naccarato R.Oxidative DNA damage in circulating leukocytes occurs as an early event in chronic HCV infection.Free Radic Biol Med 1999; 27:1284-1291
  • 8[8]Jain SK,Pemberton PW,Smith A,McMahon RF,Burrows PC,Aboutwerat A,Warnes TW.Oxidative stress in chronic hepatitis C:not just a feature of late stage disease.J Hepatol 2002; 36:805-811
  • 9[9]Parola M,Robino G.Oxidative stress-related molecules and liver fibrosis.J Hepatol 2001; 35:297-306
  • 10[10]Ramadori G,Armbrust T.Cytokines in the liver.Eur J Gastroenterol Hepatol 2001; 13:777-784

同被引文献25

引证文献5

二级引证文献25

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部