摘要
目的探讨X射线照射后鼻咽癌CNE-1细胞产生多药耐药的机制.方法采用MTr法检测X射线照射后CNE-1细胞对顺铂（cD-DP）的耐药性;采用逆转录-PCR（RT-PCR）和蛋白免疫印迹（Western blot）方法,检测照射后CNE-1细胞mdrl基因的表达.结果随照射后时间的延长,cDDP对细胞的半数抑制浓度（IC50）值逐渐增大,第2～4周IC50值显著高于照射前（P＜0.01）.照射后细胞mdrl mRNA和P-gp的表达较照射前均显著增强（P＜0.01）.结论X射线照射可诱导CNE-1细胞mdd基因的表达,这可能是照射后肿瘤细胞产生多药耐药的重要机制之一.
Objective To explore mechanism of multi-drug resistance in nasopharyngeal carcinoma （NPC） CNE-1 cells after X-ray irradiation. Methods The drug resistance of CNE-1 cells after X-ray irradiation to cisplatin （cDDP） was determined by MTY. The expression of multidrug resistance （ mdr 1 ） gene was tested by RT-PCR and Western blot. Results ICS0 values of cDDP in cells 2 ~ 4 weeks after irradiation were signiticantly higher than those before irradiation （P 〈0.01 ）. The expression of mdrl mRNA and P-gp in cells after irradiation increased than those before irradiation （P 〈 0.01 ）. Conclusions X-ray irradiation may induce the expression of mdrl gene in CNE-1 cells, which maybe play an important role in muhidrug resistance after NPC irradiation.
出处
《中国老年学杂志》
CAS
CSCD
北大核心
2006年第4期503-505,共3页
Chinese Journal of Gerontology
基金
中国科学院知识创工程领域前尚基金项目(No.DICPkaaaabc)
