摘要
M14病毒是1981年从北京郊区捕获的三带喙库蚊中分离获得。其生物学、形态学和理化特性均符合呼肠孤病毒科环状病毒的特点。聚丙烯酰胺凝胶电泳将其dsRNA基因组分离成11条区带。但最近的进一步研究发现,它是由12个片段的RNA组成。又用猴轮状病毒SA11株作为标准,测定了每个片段的分子量。 白纹伊蚊细胞C6/36纯系,由日本长畸大学热带医学研究A.Igarashi教授惠赠,并照他的方法培养传代。
It was found that the genome of orbivirus M14 contained the apparent 12-segmented dsRNA other than 11 segments as previously characterized. The molecular weights of dsRNA segments varied from 0.34×106 to 1.85× 106 daltons. The total molecular weight wes about 10.67×106 daltons. The viral dsRNA genome consisted of the medium and small segments. The present paper is important for the further studies of the viral dsRNA. The discussion how to separate RNA segment which is similar in molecular weight was made.
出处
《病毒学报》
CAS
CSCD
北大核心
1990年第2期189-191,共3页
Chinese Journal of Virology
基金
国家自然科学基金
