摘要
病样经提纯后,在电镜下获得3nm丝状和8nm分枝丝状结构,病毒样在蔗糖密度梯度离心后产生三条沉降带。经过电泳分析表明:病毒外壳蛋白为单一组分,分子量约为3.69×10~4dalton;病毒核酸有三种大小不同的组分,分子量为0.9,1.0,1.4×100dalton(混合样)。用提纯的抗原制备抗血清。提纯病毒抗原与所制备抗血清、与我国过去研究制备的抗血清及与日本制备的条纹叶枯病单克隆抗体都有血清学反应。
Rice stripe disease caused severe damage in China. In the past studies, the emphases were limited in the biological identification, epidemics and control methods, so it is still argued whether the disease iscauaed by RSV or even by a virus. In the present study, a virus with filamentous (3nm wide) or branched filamentous (8nm wide )particles were observed in purified preparations and three sedimentation components were founded with Isco fraetionor after 10—40% sucrose density gradient centrifugation. There presented asingle protein(mol, wt. 3.69×10~4)and three species of RNA(mol. wt. 0.9, 1.0, and 1.4×10~6)in preparations. With the monoclonal antibody of RSV from Japan, the virus showed a serological homology. These results certificated that the cause of rice stripe disease in China was the same RSV as that of Japan.
出处
《病毒学杂志》
CSCD
1990年第3期306-311,共6页
基金
农业部及浙江省科委经费资助
