摘要
本研究包括２个试验。试验１，采用由多种大分子物质组成的生物试剂Ｐｅｒｃｏｌ做成离心梯度，离心处理精液获得较高活率的精子。将其用于卵母细胞的体外受精，在使用４５μＬ大小的受精滴及２×１０６个／ｍＬ的最终受精精子浓度时，加入２０个或４０个卵母细胞，二者均获得较高的分裂率（％）８９．０±５．０、８８．１±３．８及囊胚率（％）５１．０±７．０、４６．３±１６．７。试验２，为了更接近胚胎在母牛体内发育的实际状态（动态），采用一种新的方法即将摇床振动培养引入牛早期胚胎的体外发育阶段，以期提高胚胎发育质量。结果表明，在早期胚胎基数及环境因素完全一致的前提下，振动培养组的囊胚发育率（％）及囊胚卵化率（％）分别为６１．１±１２．２、８２．６±６．８，而常规的静置培养组为６７．４±６．３、８８．０±７．４，方差计算表明二者无显著差异（Ｐ＞０．０５）。
Two tests were designed for improving the in vitro fertilization and in vitro development in this research. In 1st test, high ratio of live sperm can be achieved through gradient Percol centrifugation. It was possible to add more oocytes in a 45 μL fertilization drop and maintain high cleavage rate and blastoyst rate when sperm concentration was same. High cleavage rate (89.0±5.0)%, (88.1±3.8)% and blastocyst rate (51.0±7.0)%, (46.3±16.7)% were gotten when 20 or 40 oocystes were used. In 2nd test, flux culture system was used to see if it benefited embryo development. The blastoyst rate of flux culture and static culture were (61.1±12.2)% and (67.4±6.3)% respectively. The hatched blastoyst rates of two groups were (82.6±6.8)% and (88.0±7.4)%. No significant difference existed between two culture systems.
出处
《中国兽医学报》
CAS
CSCD
1996年第5期510-513,共4页
Chinese Journal of Veterinary Science
关键词
牛
卵母细胞
振动培养
体外受精
体外发育
bovine oocyte
gradient Percol centrifugation
flux cultivation
in vitro fertilization
in vitro development
