摘要
将200009批疫苗分三个剂量组(Ⅰ,Ⅱ,Ⅲ),病毒含量分别为每羽份含500、1000、1500EID50,口服免疫13周龄SPF鸡,同时设进口同类疫苗组(Ⅳ)和未接种空白组(V)作为对照。分别于免疫后7d、14d、17d、21d、28d、42d、56d、70d、84d、105d、126d、149d、169d采血,分离血清,采用IDEXX禽脑脊髓炎抗体检测ELISA试剂盒检测血清中AEV抗体水平,并通过鸡胚易感试验和后代雏鸡试验评价产蛋后鸡群的免疫状态。结果表明,疫苗接种后14d,免疫鸡血清已全部阳转,免后42d时抗体出现第一个峰值,此后一直保持较高水平,并且抗体水平的高低与接种疫苗的病毒含量呈正相关关系。用AEVVR强毒进行的鸡胚易感试验,免后6～55周免疫组鸡胚的感染率在35%以下;后代雏鸡攻毒保护率在92.9%以上。
Thirteen-week-old SPF chickens were divided into five groups,the group Ⅰ,Ⅱ,Ⅲ were inoculated by oral administration with avian encephalomyelitis living vaccine （lot 200009） contained different antigen concentration （500, 1000, 1500 EID50/dose）,respectively, meanwhile, the group Ⅳ was inoculated with imported same AE vaccine and the group Ⅴ was as blank control. On the day of 7, 14, 17, 21, 28, 42, 56, 70, 84, 105, 126, 149 and 169 post inoculation, blood samples were taken, and the titers of antibody against AEV were detected by ELISA Kit（IDEXX）. The results shown that all of inoculated chickens＇ blood samples were antibody positive at 14th day, and antibody level would be reached the first peak on 42nd day post inoculation, then remained higher level later. There was a positive relation between the antibody titer and antigen concentration in vaccine. The challenge results of the 14-day-age progeny chickens inoculated with virulent AEV VR strain is the same as changes of Embryo- sensitivity test. The infected rate of immune chickens was below 35 %. The vaccine could offer more than 92.9 % protection at least 12 months.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2006年第3期347-350,共4页
Chinese Journal of Preventive Veterinary Medicine
关键词
禽脑脊髓炎
活疫苗
免疫持续期
母源抗体
avian encephalomyelitis
living vaccine
immtme duration
maternal antibody
