摘要
目的探讨蝉翼藤醇提取物对小鼠淋巴细胞体外增殖和分泌白细胞介素-2(IL-2)与肿瘤坏死因子β(TNF-β)的影响。方法向小鼠脾细胞加入刀豆蛋白(ConA)和蝉翼藤醇提取物进行培养,以MTT法检测淋巴细胞增殖反应,用ELISA法测定IL-2和TNF-β分泌水平。结果蝉翼藤醇提取物能增强ConA诱导的小鼠脾细胞的体外增殖和分泌IL-2和TNF-β的水平。结论蝉翼藤醇提取物对小鼠淋巴细胞活性有增强作用。
Objective To study the effects of ethanol extracts of a Chinese medical herb stem and radix of Securidaca inappendiculata lassk （CYT）, on lymphocyte proliferation and secretion of interleukin-2 （IL-2） and tumor necrosis factor （TNF-β ） of mouse spleen cells in vitro. Methods Different concentrations of ethanol extracts of CYT were added to mouse spleen cell cultures supplemented with ConA. Lymphocyte proliferation reaction was detected by MTT assay. IL-2 and TNF-β secreting level were measured by ELISA. Results Ethanol extract of Securidaca inappendiculata Hassk increased cultured mouse spleen cell proliferation and secretion of IL-2 and TNF-β in the presence of ConA. Conelusion Ethanol extracts of CYT could raise mouse lymphocyte activities in vitro.
出处
《中国中医药信息杂志》
CAS
CSCD
2006年第5期42-43,共2页
Chinese Journal of Information on Traditional Chinese Medicine
